LOCUS       pHR15_for_Chlamy        7772 bp    DNA     linear       13-MAY-2014
DEFINITION  pHR15 plasmid.
ACCESSION   urn.local...1400018715922.41
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     This file is created by Vector NTI http://www.invitrogen.com/
            VNTDATE|605717957| VNTDBDATE|605725390| LSOWNER| VNTNAME|pHR10_Hcr1
            HSP70RBCS2-ARScDNA-Ars3UTR with 2kb intron| VNTAUTHORNAME|Hussam H
            Nour Eldin|.
FEATURES             Location/Qualifiers
     3'UTR           4802..5446
                     /created_by="User"
                     /Transferred_From="ARS2 full gene from phytozome, pJD100"
                     /Transferred_Similarity="100.00%"
                     /vntifkey=50
                     /modified_by="User"
                     /label="Ars2 3' UTR"
     CDS             complement(6663..7520)
                     /vntifkey=4
                     /modified_by="User"
                     /label="amp res b-lactamase"
     misc_feature    2161..2163
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    156..>2159
                     /Original_Bases
                     /modified_by="User"
                     /label="Paro cassette"
     promoter        2470..2851
                     /vntifkey=29
                     /modified_by="User"
                     /label="RbcS2"
     promoter        2179..2451
                     /vntifkey=29
                     /label="HSP70"
     exon            4061..4262
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#11"
     exon            4526..4711
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#14"
     exon            3355..3528
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#5"
     exon            3091..3241
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#3"
     exon            4263..4411
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#12"
     exon            3529..3675
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#6"
     exon            2954..3090
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#2"
     exon            3839..3961
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#9"
     exon            4412..4525
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#13"
     exon            3242..3354
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#4"
     exon            2852..2953
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="ARS2 full gene from phytozome, pJD100"
                     /Transferred_Similarity="100.00%"
                     /vntifkey=61
                     /label="first\coding\exon\of\ARS2\gene"
     exon            3962..4060
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#10"
     exon            3676..3767
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#7"
     exon            4712..4801
                     /created_by="User"
                     /Transferred_From="ARS2 full gene from phytozome, pJD100"
                     /Transferred_Similarity="100.00%"
                     /vntifkey=61
                     /label="last\coding\exon\of\Ars2"
     exon            3768..3838
                     /created_by="User"
                     /vntifkey=61
                     /label="exon\#8"
ORIGIN      
        1 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
       61 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
      121 gagagtgcac catagggcgg ccgccagctg gaattcatcc cacacacctg cccgtctgcc
      181 tgacaggaag tgaacgcatg tcgagggagg cctcaccaat cgtcacacga gccctcgtca
      241 gaaacacgtc tccgccacgc tctccctctc acggccgacc ccgcagccct tttgcccttt
      301 cctaggccac cgacaggacc caggcgctct cagcatgcct caacaacccg tactcgtgcc
      361 agcggtgccc ttgtgctggt gatcgcttgg aagcgcatgc gaagacgaag gggcggagca
      421 ggcggcctgg ctgttcgaag ggctcgccgc cagttcgggt gcctttctcc acgcgcgcct
      481 ccacacctac cgatgcgtga aggcaggcaa atgctcatgt ttgcccgaac tcggagtcct
      541 taaaaagccg cttcttgtcg tcgttccgag acatgttagc agatcgcagt gccacctttc
      601 ctgacgcgct cggccccata ttcggacgca attgtcattt gtagcacaat tggagcaaat
      661 ctggcgaggc agtaggcttt taagttgcaa ggcgagagag caaagtggga cgcggcgtga
      721 ttattggtat ttacgcgacg gcccggcgcg ttagcggccc ttcccccagg ccagggacga
      781 ttatgtatca atattgttgc gttcgggcac tcgtgcgagg gctcctgcgg gctggggagg
      841 gggatctggg aattggaggt acgaccgaga tggcttgctc ggggggaggt ttcctcgccg
      901 agcaagccag ggttaggtgt tgcgctcttg actcgttgtg cattctagga ccccactgct
      961 actcacaaca agccaaaatg gacgatgcgt tgcgtgcact gcggggtcgg tatcccggtt
     1021 gtgagtgggt tgttgtggag gatggggcct cgggggctgg tgtttatcgg cttcggggtg
     1081 gtgggcggga gttgtttgtc aaggtggcag ctctgggggc cggggtgggc ttgttgggtg
     1141 aggctgagcg gctggtgtgg ttggcggagg tggggattcc cgtacctcgt gttgtggagg
     1201 gtggtgggga cgagagggtc gcctggttgg tcaccgaagc ggttccgggg cgtccggcca
     1261 gtgcgcggtg gccgcgggag cagcggctgg acgtggcggt ggcgctcgcg gggctcgctc
     1321 gttcgctgca cgcgctggac tgggagcggt gtccgttcga tcgcagtctc gcggtgacgg
     1381 tgccgcaggc ggcccgtgct gtcgctgaag ggagcgtcga cttggaggat ctggacgagg
     1441 agcggaaggg gtggtcgggg gagcggcttc tcgccgagct ggagcggact cggcctgcgg
     1501 acgaggatct ggcggtttgc cacggtcacc tgtgcccgga caacgtgctg ctcgaccctc
     1561 gtacctgcga ggtgaccggg ctgatcgacg tggggcgggt cggccgtgcg gaccggcact
     1621 ccgatctcgc gctggtgctg cgcgagctgg cccacgagga ggacccgtgg ttcgggccgg
     1681 agtgttccgc ggcgttcctg cgggagtacg ggcgcgggtg ggatggggcg gtatcggagg
     1741 aaaagctggc gttttaccgg ctgttggacg agttcttctg agcgttctgg cagcagctgg
     1801 accgcctgta ccatggagaa gagctttact tgccgggatg gccgatttcg ctgattgata
     1861 cgggatcgga gctcggaggc tttcgcgcta ggggctaggc gaagggcagt ggtgaccagg
     1921 gtcggtgtgg ggtcggccca cggtcaatta gccacaggag gatcaggggg aggtaggcac
     1981 gtcgacttgg tttgcgaccc cgcagttttg gcggacgtgc tgttgtagat gttagcgtgt
     2041 gcgtgagcca gtggccaacg tgccacaccc attgagaaga ccaaccaact tactggcaat
     2101 atctgccaat gccatactgc atgtaatggc caggccatgt gagagtttgc cgtgcctgga
     2161 attcgctgag ggtttaatcg gcggggagct cgctgaggct tgacatgatt ggtgcgtatg
     2221 tttgtatgaa gctacaggac tgatttggcg ggctatgagg gcgggggaag ctctggaagg
     2281 gccgcgatgg ggcgcgcggc gtccagaagg cgccatacgg cccgctggcg gcacccatcc
     2341 ggtataaaag cccgcgaccc cgaacggtga cctccacttt cagcgacaaa cgagcactta
     2401 tacatacgcg actattctgc cgctatacat aaccactcag ctagcttaag atcccatcaa
     2461 gcttgcatgc cgggcgcgcc agaaggagcg cagccaaacc aggatgatgt ttgatggggt
     2521 atttgagcac ttgcaaccct tatccggaag ccccctggcc cacaaaggct aggcgccaat
     2581 gcaagcagtt cgcatgcagc ccctggagcg gtgccctcct gataaaccgg ccagggggcc
     2641 tatgttcttt acttttttac aagagaagtc actcaacatc ttaaaatggc caggtgagtc
     2701 gacgagcaag cccggcggat caggcagcgt gcttgcagat ttgacttgca acgcccgcat
     2761 tgtgtcgacg aaggcttttg gctcctctgt cgctgtctca agcagcatct aaccctgcgt
     2821 cgccgtttcc atttgcagga tggccatgca tatgggtgcc ctcgcggtgt tcgccgtcgc
     2881 ttgcctcgcg gcagtggcgt cggttgcgca tgcggccgac accaaaaagc ccaactttgt
     2941 ggtgatcttc accgatgacc aggacgccat tcagaacagc acccacccgc actacatgcc
     3001 cagcctgcac aagtacatcc gctacccggg agtggagctg tctcagtact tcgtcaccac
     3061 ccccgtgtgc tgcccctcgc ggacaaacct gtggcgcggc cagttcgccc acaacaccaa
     3121 cttcaccagc gtgctgcctc cctacggtgg ctgggccaag tggaagggcc tgggcatcga
     3181 ccagtcctac ctgccgctgt ggctcaagga ccaaggctat aacacctact acgtgggcaa
     3241 gttccttgtg gactactcgg tcagcaacta ccagcaggtg cccgcgggct gggacgacat
     3301 cgatgccctg gtcaccccct acacctttga ctacaacacc cccggcttca gccgcaacgg
     3361 cgcgaccccc aacatctacc ccggcgagta cagcactgac gtcattcgcg acaagggcat
     3421 tgctcagatc aagtcggccg tggctgccgg aaagcccttc tacgcgcaga tctcgcccat
     3481 cgcgccgcac acctccaccc agatttccac cgaccccgtc accggagtga cgaggtcctt
     3541 cttctacccg cccatccccg ccccccgcca ctggcagctg ttctccgacg ccaacctgcc
     3601 cggcggcacg cccaacaaga acctgtacga ggtggacgtg agcgacaagc ccgcctgggt
     3661 ccgcgccctg ccgctggccc agcagaacaa ccgcacctac ctggaggaga tctaccgcct
     3721 gcgcctgagg tcgctggcgg ccgtggacga gctgattgag caagtcgtca agaccctgga
     3781 tgaggcgggt gtgcttgaca acacctacat catctacagc gctgacaacg gctaccacgt
     3841 gggtgcccac cgcttcggcg cgggcaagac cacgggctat gaggaggacc tgcgtgtgcc
     3901 cttcctcatc cgcggcccag gcatcaaggc cagccagtcc gacaagccgc agaacagcaa
     3961 ggttggcctg cacgtggact ttgcgcccac cattctcagc ctggccggcg cctcgcacct
     4021 gctcggggac aaggggctgg acggcacccc gctgggcctg tacgccaacg acgacggcac
     4081 tcttcgctcc gactaccctc gtccggagca gcaccgccag cagttccagg gcgagttctg
     4141 gggcggctgg agtgatgagc tgctgcagaa cctcaggtcc cagcccaaca acacttggaa
     4201 ggtggtgcgc acgtatgacg agagcagcaa gcagggatgg aagctcatcg cgcagtgcac
     4261 caacgagcgc gagctgtacg acctgcgcaa ggaccccggt gagctgtaca acatctacga
     4321 caaggccaag cccgccgtgc gcagccgcct ggaggggctg ctggcggtgc tggccgtgtg
     4381 caagggggag agctgctcca acccgtggaa gatcctgcac cccgacggca ccgtcaagaa
     4441 cttcacccag gcactcaact ccaagtacga ccgcatctac aacgcaatcc gccccttcac
     4501 ctacaagacg tgcctgcagt acctggattg ggacaacgag gacagtcagt ttaagacgca
     4561 gatccgcggc gccaaccccg cagccggcgt gggccaccac cgcctgctca ccgccgccag
     4621 cgagcgcgcc atcgccaccc gccgccgcgc ccaggccgcc gtcagtgccg agctggcgga
     4681 gcggccggct gtgttccagg caaaggtcga ggagaagtcg gtgccggtgc cccaggacat
     4741 cctgaaggcc gacgtggaga agtggttcgc cttcaacaat gccgagtact acctggctta
     4801 gatggtcgat attatataaa agccaatgca agcgcgcatg gacatagcgc atcgaccaag
     4861 cgccaccatg gcttgggttt ctttgatacg gttgggctaa gtttgatatg tgggttttgg
     4921 acgtggccgc ttggtcagta agcggtccac gtggtaatgc cgtgtgcgtg atcccccctg
     4981 gagtggtgtt ggggtaggtt aatgacaagg taaagcagtg ggtacatgca cgcacaattg
     5041 cgtcggacag aagagtaccg ggacgtgatc catgaagaaa tggtataagg cgcctcatgc
     5101 atccgtagat ggcgctcacg tgcgcttaat tgcatgcgcg ccgtcacttg tttgttgatt
     5161 gcggaattaa gtggttaggc cacttggttg cgaagagtgt tgtgcgccgc cctcggtagt
     5221 tcggtgcgcc gctggaaact tgcgttggtg ttctgagctg cggagctctg gttggtcact
     5281 tggtctgctg ttgtgcctgt atgttaagag gtgctgggta aagaagtggg cttgcgtgga
     5341 tgttgactgg ctggcagata ggactgtgca gcggccttgc tgccgcgtgg taaagactga
     5401 gaaaggtatg tacccggcgt ggtgccatgg agccatggaa cgaagcatta agacctcagc
     5461 ctgcagagta ctgcggccgc gagcttggcg taatcatggt catagctgtt tcctgtgtga
     5521 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5581 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5641 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5701 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6301 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7621 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     7681 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     7741 ggtgaaaacc tctgacacat gcagctcccg ga
//
