* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $1000.00. Subscriptions cannot be used for CLiP mutants, BACs nor fosmids.
pASapI
$30.00
From Saul Purton, University College London, August 2014
Chloroplast DNA sequence containing psbH-trnE2 region with an ‘expression cassette’ engineered into the intergenic MluI site. Cassette comprises of the atpA promoter/5’UTR and rbcL 3’UTR regions separated by a multiple cloning site. Note: the SapI site in the MCS is designed to allow a perfect translational fusion at the ATG of the gene-of-interest.
host strain: DH5α
amp resistant