pOpt_mVenus_Hyg
$30.00
From Kyle J. Lauersen, Jan H. Mussgnug, Olaf Kruse, Center for Biotechnology (CeBiTec), Bielefeld University, Germany, June 2014
NCBI accession number: KM061065
Vector: pBluescript backbone, pOptimized synthetic Chlamydomonas expression cassettes
Origin: synthetic construct (DNA synthesis and cloning)
Insert: pOptimized template vector containing the mVenus (YFP variant) reporter expression cassette and an antibiotic selection marker expression cassette. Both cassettes contain the HSP70A-RBCS2-InI promoter and RBCS2 3’UTR surrounding either the reporter or the marker. Each reporter contains intron 2 of RBCS2 within its nuclear sequence. Selection marker for Chlamydomonas is hygromycin B resistance aphVII.
Host strain: DH5α
Selection: Ampicillin resistance for E. coli culture
Comment: Please follow naming convention for derivative vectors using underscores between new added elements.
Individual annotated vector maps are available in ApE format.