pOpt_mVenus_Hyg
$30.00
From Kyle J. Lauersen, Jan H. Mussgnug, Olaf Kruse, Center for Biotechnology (CeBiTec), Bielefeld University, Germany, June 2014
NCBI accession number: KM061065
Vector: pBluescript backbone, pOptimized synthetic Chlamydomonas expression cassettes
Origin: synthetic construct (DNA synthesis and cloning)
Insert: pOptimized template vector containing the mVenus (YFP variant) reporter expression cassette and an antibiotic selection marker expression cassette. Both cassettes contain the HSP70A-RBCS2-InI promoter and RBCS2 3’UTR surrounding either the reporter or the marker. Each reporter contains intron 2 of RBCS2 within its nuclear sequence. Selection marker for Chlamydomonas is hygromycin B resistance aphVII.
Host strain: DH5α
Selection: Ampicillin resistance for E. coli culture
Comment: Please follow naming convention for derivative vectors using underscores between new added elements.
Individual annotated vector maps are available in ApE format.
Lauersen KJ, Kruse O, Mussgnug JH (2015) Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit. Appl Microbiol Biotechnol. Jan 15 Lumbreras V, Purton S (1998) Recent advances in chlamydomonas transgenics. Protist 149:23-7 Berthold P, Schmitt R, Mages W (2002) An engineered Streptomyces hygroscopicus aph 7" gene mediates dominant resistance against hygromycin B in Chlamydomonas reinhardtii. Protist 153:401-12 Sizova I, Fuhrmann M, Hegemann P (2001) A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii. Gene 77:221-9