* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $1000.00. Subscriptions cannot be used for CLiP mutants, BACs nor fosmids.
pHS-SpCas9 (pPH185)
$30.00
From Simon Kelterborn, Peter Hegemann lab, Humboldt University-Berlin October 2017
Expression of Streptococcus pyogenes (Sp)Cas9 optimized to C. reinhardtii codon bias, controlled by the HSP70A heat-shock promoter.
Host strain: XL1-Blue
Selection in E.coli: ampicillin
Link to sequence and map
Sequence (.docx file)
Overview of all CRISPR/Cas9 plasmids from the Hegemann lab
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de