• Strain #:
  • CC-110 spr-u-1-6-2 mt+
  • Description:

  • Boynton-Gillham laboratory, Duke University

    Phenotype: antibiotic resistant (spectinomycin)

    This strain was derived at Duke from a cross between CC-147 nr1-8 spr-u-1-62 and CC-198 er-u-37 sr-u-2-60; it does not carry nr1-8 or the markers from CC-198.

    This is a good chloroplast resistance marker, easy to score on 100 microgram/ml spectinomycin. spr-u-1-6-2 is an A to G mutation at position 1123 in the chloroplast 16S rRNA and confers high level spectinomycin resistance under all growth conditions.

    Harris et al. (1989) Genetics 123, 281-292

  • Chromosome:

  • chloroplast

  • Locus:

  • rrnS

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