• Strain #:
  • CC-118 sr-u-2-60 mt+
  • Description:

  • Boynton-Gillham laboratory, Duke University

    Phenotype: antibiotic resistant (streptomycin)

    CC-118 was derived from a cross of wild type mt+ x sr-u-2-60 mt- (equivalent to CC-119).

    This is an excellent chloroplast mutant, easy to score as resistant to up to 500 micrograms/ml streptomycin on agar (50-100 micrograms is sufficient to kill wild type cells). The mutation is an A to C change at base 474 of 16S rRNA gene (equivalent to E. coli 523).

    Harris et al. (1989) Genetics 123, 281-292

  • Chromosome:

  • chloroplast

  • Locus:

  • rrnS

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