• Strain #:
  • CC-43 ac20 mt-
  • Description:

  • Boynton-Gillham laboratory, Duke University

    Phenotype: cold sensitive on minimal medium

    Product 5.6.1 from a cross of wild type to the original ac20 cr1 double mutant (CC-154 mt-, a strain now discontinued.)

    This strain was used by Boynton et al. (1972) in their study of mutants deficient in chloroplast ribosomes, and defines the ac20 mutation in terms of chloroplast ribosome content (deficient in both ribosomal subunits). Like other mutants deficient in chloroplast ribosomes, ac20 is easiest to score by slow growth on minimal medium at cold temperature (15-17 degrees). Considered purely as a genetic marker, ac20 is not the best choice for this region of linkage group I. However, CC-43 appears to be a stable strain with the correct ac20 phenotype.

    Herrin and colleagues determined that ac20 overaccumulates an unspliced 23S pre-RNA in the chloroplast, and that the primary defect in the mutant is in ITS processing rather than splicing.

    Togasaki and Levine (1970) J. Cell Biol. 44, 531-539
    Levine and Paszewski (1970) J. Cell Biol. 44, 540-546
    Goodenough and Levine (1970) J. Cell Biol. 44, 547-562
    Boynton et al. (1972) J. Cell Sci. 10, 267-305
    Harris et al. (1974) J. Cell Biol. 63, 160-179
    Herrin et al. (1990) J. Biol. Chem. 265, 21134-21140
    Thompson and Herrin (1991) Nucl. Acids Res. 19, 6611-6618
    Holloway and Herrin (1998) Plant Cell 10, 1193-1206

  • Chromosome:

  • 01

  • Locus:

  • AC20

  • $10.00