• Strain #:
  • CC-48 arg2 mt+
  • Description:

  • Boynton-Gillham laboratory, Duke University

    Phenotype: requires arginine

    CC-48 came from R.P. Levine, and was brought to Duke University by N.W. Gillham in 1968. This is a stable arginine-requiring strain, easy to score.

    The arg2 mutation, which was UV-induced by Eversole, is an allele at the ARG7 locus, encoding argininosuccinate lyase, and is also known as arg7-8. These two mutations are capable of intragenic complementation, and are often used to create diploid strains. Diploids formed between arg2 and the original arg7 mutant are stable and grow at wild type rates in the absence of arginine. Intragenic complementation is also seen between arg2 and arg7-2, arg7-3, arg7-4, arg7-5, arg7-7, and arg7-12 (see Loppes et al., 1972, and Matagne, 1978).

    The wild type gene at this locus was cloned by Debuchy et al., and used to transform arg7 mutants to arginine independence.

    The arg2 and arg7 mutants require arginine (50 micrograms per ml is recommended) and cannot use ornithine or citrulline. They also grow well on medium containing yeast extract (4 grams per liter).

    The following references pertain to arg2 and to other alleles at the ARG7 locus:

    Eversole (1956) Amer. J. Bot. 43, 404-407
    Ebersold and Levine (1959) Z. Vererbs. 90, 74-82
    Hudock (1962) Biochem. Biophys. Res. Commun. 9, 551-555
    Hudock (1963) Biochem. Biophys. Res. Commun. 10, 133-138
    Gillham (1965) Genetics 52, 529-537
    Loppes (1966) Z. Vererbs. 98, 193-202
    Loppes (1969) Mol. Gen. Genet. 104, 172-177
    Strijkert and Sussenbach (1969) Eur. J. Biochem. 8, 408-412
    Strijkert and Sussenbach (1969) Eur. J. Biochem. 8, 408-412
    Sussenbach and Strijkert (1969) Eur. J. Biochem. 8, 403-407
    Loppes (1970) Experientia 26, 660-661
    Levine and Goodenough (1970) Annu. Rev. Genet. 4, 397-408
    Loppes and Matagne (1972) Genetica 43, 422-430
    Loppes and Strijkert (1972) Mol. Gen. Genet. 116, 248-257
    Loppes et al. (1972) Heredity 28, 239-251
    McMahon and Langstroth (1972) J. Gen. Microbiol. 73, 239-250
    Strijkert et al. (1973) Biochem. Genet. 8, 239-248
    Konvalinkova et al. (1974) Mut. Res. 24, 69-72
    Schimmer and Loppes (1975) Mol. Gen. Genet. 138, 25-31
    Matagne (1976) Mol. Gen. Genet. 146, 209-214
    Matagne (1978) Mol. Gen. Genet. 160, 95-99
    Matagne and Vincenzotto (1979) Arch. Int. Physiol. Biochim. 87, 832-833
    Debuchy et al. (1989) EMBO J. 8, 2803-2809
    Gumpel et al. (1994) Trends Cell Biol. 4, 299-301

  • Chromosome:

  • 01

  • Locus:

  • ARG7

  • $10.00