CC-4677 16-5K pf2 mt+ (pmp1, ad, LCIB) pf2 progeny used for genetic analysis
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires elevated CO2 for photosynthetic growth
Mutant 16-5K mt+ (CC-1860) was crossed with pf2 mt-, and this pf2 progeny clone was retained for further genetic analysis. Mutant 16-5K was originally recovered as an acetate-requiring mutant (Spreitzer and Ogren 1983). Subsequent screening found it to be rescued by elevated CO2, and deficient in inorganic carbon transport (Spalding et al. 1983). This strain has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Spalding MH, Spreitzer RJ, Ogren WL (1983) Reduced inorganic carbon transport in a CO2-requiring mutant of Chlamydomonas reinhardii. Plant Physiol 73:273-276
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