CC-4791 rbcL-G54D mt+ (31-4E) (non-pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate, sensitive to light
The 31-4E chloroplast mutant was recovered by screening a collection of acetate-requiring mutants (generated with 5-fluorodeoxyuridine and methyl methanesulfonate) for lack of complementation with a known Rubisco rbcL mutant by standard methods (Spreitzer and Ogren 1983; Spreitzer et al. 1988). The mutant was crossed with pf2 mt- to confirm uniparental inheritance, and this mt+ progeny clone was analyzed. Mutant 31-4E results from a G54D substitution (GGT-GAT) in the chloroplast-encoded large subunit of Rubisco, which blocks Rubisco holoenzyme assembly (Spreitzer 1993). Selection for photosynthesis-competent revertants yielded true revertants and pseudorevertants (see R95-1C and R95-10A) (Spreitzer et al. 1995). This strain has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ (1993) Genetic dissection of Rubisco structure and function. Annu Rev Plant Physiol Plant Mol Biol 44:411-434
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777
Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Spreitzer RJ, Thow G, Zhu G (1995) Pseudoreversion substitution at large-subunit residue 54 influences the CO2/O2 specificity of chloroplast ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 109:681-686
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