* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
P-260 cpDNA KpnI (3.1 kb) DCMU4
$30.00
Boynton-Gillham laboratory, Duke University, 1990
Chloroplast DNA KpnI (3.1 kb) in BSKS+; contains psbA exons 4 and 5 from DCMU4 mutant, CC-2473
host strain: JM109
amp resistant
More information
Strain: CC-2473 DCMU4
Insert: KpnI (3.1 kb)
Genes present: psbA exons 4 and 5
Vector: BSKS+
Bacterial host strain: JM109
Selectable marker: amp-r, white on X-gal
Origin: Amnon Lers in Boynton/Gillham lab, 1990
DCMU4 is a ser->ala mutation at aa 264 in the C. reinhardtii psbA 5th exon, conferring resistance to DCMU and atrazine. The insert was subcloned into the KS+ KpnI site in both orientations. In P-260A, the psbA direction is from the KS SacI site towards the KpnI site; in P-260B, it is from the KS KpnI site towards the SacI site.