* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $1000.00. Subscriptions cannot be used for CLiP mutants, BACs nor fosmids.
pALM30-CrSpS
$30.00
Source: Olivier Vallon, Institut de Biologie Physico-Chimique, Paris, November 2011
Used to modify Chlamydomonas genomic BACs to Sp resistance
Insert: CrAadA (AR promoter, RBCS2 3′-UTR)
Origin: pCVD442 with IS1 and CmR removed (NdeI AsiSI) and replaced by bla, then inserting 3 kbp of pBeloBAC11-Nit8 (AscI AgeI), then inserting (FseI) the CrAadA marker from pALM32 (antisense with NIT8)
Vector: R6K
Host strain: S17-1 λpir
Amp resistant
