TAP and Tris-minimal* recipes
[Gorman, D.S., and R.P. Levine (1965) Proc. Natl. Acad. Sci. USA 54, 1665-1669]
Stock solutions for 1 L of TAP media (adjust final pH to 7.0)
1M Tris base (e.g. Trizma) 20 ml
Phosphate Buffer II 1.0 ml
Solution A 10.0 ml
Hutner’s trace elements 1.0 ml
Glacial acetic acid 1.0 ml
*For Tris-minimal medium omit the acetic acid and titrate the final solution to pH 7.0 with HCl
Phosphate Buffer II (for 100 ml)
K2HPO4 10.8 g
KH2PO4 5.6 g
Solution A (for 500 ml)
NH4Cl 20 g
MgSO4-7H2O 5 g
CaCl2-2H2O 2.5 g