CC-1388 vfl1 mt+
$30.00
From David Luck, Rockefeller University, February 1983
Phenotype: variable number of flagella; abnormal cell shape; abnormal cell division
Mutants at the VFL1 locus typically have between 0 and 4 flagella, sometimes as many as 10. Cell size and shape are often abnormal, probably as a result of unequal cell divisions.. Flagellar assembly continues throughout the G1 portion of the cell cycle. This feature is unique to vfl cells.
Flagella can appear at any site on the cell surface, and there may be defects in the flagellar apparatus, including missing or defective striated fibers and reduced numbers of rootlet microtubules. Wright et al. determined that, in contrast to vfl2 mutants, vfl1 has normal levels of centrin and a stable nucleus-basal body connector.
Adams GM, Wright RL, Jarvik JW (1985) Defective temporal and spatial control of flagellar assembly in a mutant of Chlamydomonas reinhardtii with variable flagellar number. J Cell Biol 100:955-964
Wright RL, Adler SA, Spanier JG, Jarvik JW (1989) Nucleus-basal body connector in Chlamydomonas: evidence for a role in basal body segregation and against essential roles in mitosis or in determining cell polarity. Cell Motil Cytoskeleton 14:516-526
Tam LW, Lefebvre PA (1993) Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis. Genetics 135:375-384
Silflow CD, LaVoie M, Tam LW, Tousey S, Sanders M, Wu W, Borodovsky M, Lefebvre PA (2001) The Vfl1 Protein in Chlamydomonas localizes in a rotationally asymmetric pattern at the distal ends of the basal bodies. J Cell Biol 153:63-74