* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $1000.00. Subscriptions cannot be used for CLiP mutants, BACs nor fosmids.

CSI_FC2B08

$30.00

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre10.g427250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

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CSI_FC2B08

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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