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CSI_LW02D2

$30.00

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre16.g664801-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: RBL4

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CSI_LW02D2

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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