CSI_LW01G10
$30.00
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g544114-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: CYN20-6