Release of version 3.0 of the C. reinhardtii genome sequence
Dear Colleagues, I am pleased to announce that JGI has completed automated annotation of Chlamydomonas reinhardtii v3.0. 15,256 gene models have been predicted and annotated; over 2,000 curated annotations have been transferred from the previous release. Genome Portal is now publicly available from www.jgi.doe.gov/chlamy Best regards, Igor Grigoriev Genome Annotation Group Leader US DOE Joint … Continued
Chlamydomonas flagellar proteome database
The flagellar proteome database described by Pazour et al, J. Cell Biol. 170, 103-113 (2005) is now on line at http://labs.umassmed.edu/chlamyfp. The website has a sortable list of all proteins identified in C. reinhardtii flagellar membrane, matrix and axonemal fractions by mass spectrometry. The list can be searched by Interpro descriptions or for transmembrane helices, … Continued
Molecular Markers for Map-Based Cloning
One of the goals of the current Chlamydomonas genome project is to saturate the genome with molecular markers, which can be used for map-based cloning or other genetic studies. Thanks to earlier work from the Mets, Dutcher and Silflow/Lefebvre laboratories, supplemented by our own, we compiled a list of 526 markers as recently published in … Continued
Version 2 Microarray
The version 2 microarray is ready for shipping. This array consists of about 10,000 oligonucleotides, each approximately 70 nucleotides long. Each of the oligonucleotide should represent a unique gene. The set of 10,000 oligonucleotides was printed twice on the same slide (so each slide has duplicates). The oligonucleotides were prepared by Integrated DNA Technologies and … Continued