Now Available from the Chlamydomonas Resource Center: the Chlamydomonas Fosmid Collection

You may have noticed when browsing at the Chlamydomonas site on Phytozome (https://phytozome.jgi.doe.gov/pz/portal.html#!info?alias=Org_Creinhardtii) that almost every part of the genome is covered by multiple fosmid clones, defined by the end sequences (“VTP sequences”). To see these alignments check the “BLASTN-aligned BAC and fosmid end reads” under “alignments” on the left side of the browser. The … Continued

Efficient CRISPR-based method for targeted disruption of genes in Chlamydomonas

In May 2020, the Witman and Oda labs published a paper titled “TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii” (PMC7219734).  The paper describes an easy, rapid, and cost-effective method for efficiently targeting specific genes for disruption in Chlamydomonas.  The authors tested TIM on six different genes in two different cell-walled strains … Continued

Postdoc position available in the George Witman lab at UMass Medical School

An NIH-funded postdoctoral position currently is available in the Witman lab.  We study cilia and flagella (terms here used interchangeably) to understand how they are assembled and function and why defects in specific ciliary proteins cause human diseases.  Most of our research utilizes Chlamydomonas reinhardtii, which has major advantages for studies of flagella; we also … Continued

New opportunity for screening the CLiP collection

In response to a number of requests from users, the Chlamydomonas Resource Center is offering users a chance to screen a large collection of the most valuable mutants in the Jonikas CLiP library for traits of interest. The B-Series CLiP Collection (called the B-series for “Best”), consists of more than 13,000 mutants selected as having … Continued

Postdoc position available in the Jonikas lab at Princeton University

Photosynthetic organisms, our planet’s greatest chemists, form the foundations for human health by providing us with food, fuel, materials and drugs. Yet, our understanding of these organisms is in its infancy. The Jonikas laboratory aims to enable advances in sustainability by developing transformative tools and resources for photosynthetic organisms. We currently seek an outstanding postdoctoral … Continued

Postdoc position available in the Onishi lab at Duke University

https://academicjobsonline.org/ajo/Duke/Biology/MOPD The Onishi lab (https://sites.duke.edu/onishilab) in the Duke University Department of Biology (Durham, North Carolina, USA) is looking for a Postdoctoral Researcher to join our team to work on a project to investigate the fundamental core mechanisms of eukaryotic cytokinesis and their evolution.  Our current primary model for this work is the green alga Chlamydomonas … Continued