We’d like to add more files to this page – if you have suggestions, or files you can contribute, please write us: chlamy@umn.edu
- Recipes for culture media
- TAP, HS, Sager and Granick, 6xP, Hutner’s & Kropat’s trace elements
- Chlamydomonas zone inhibition assay – without top agar plates (downloadable file)
- from Nitya Subrahmanian and Andrew Castonguay
- 10-fold dilution series for assessing Chlamydomonas growth deficiencies (downloadable file)
- from Nitya Subrahmanian and Andrew Castonguay
- Triple cocktail antibiotic plates (downloadable file)
- from Nitya Subrahmanian and Andrew Castonguay
- Mating and tetrad separation of Chlamydomonas reinhardtii for genetic analysis
- video by Xingshan Jiang and David Stern, published on-line, Journal of Visualized Experiments, August 12, 2009; PMID: 19684568
- Laboratory protocols for molecular genetics of Chlamydomonas
- EMBO Practical Course: Molecular Genetics of Chlamydomonas, Laboratory Protocols, 4-9 (2006)
- ARG7 and Ble Marker for Nuclear Transformation
- Saul Purton’s page
- Hygromycin Resistance Vectors for Nuclear Transformation
- from Peter Berthold and Wolfgang Mages
- Antibodies
- For information on commercially available antibodies to Chlamydomonas proteins, please contact Dr. Joanna Porankiewicz-Asplund
- Quick and Easy Genomic DNA Prep for PCR Analysis
- from Steve Pollock
- Cleaning Up Contaminated Cultures
- discussion from bionet.chlamydomonas, several contributors
- Isolating Chlamydomonas from Soil Samples
- from Cliff Zeyl
- Freezing Chlamydomonas Cells
- from Dick Sayre; method involves methanol as cryoprotectant and storage in liquid nitrogen, the most effective protocol as yet developed
- Encouraging Chlamydomonas Strains to Mate
- from various contributors
- Mating Type Tests in Multiwell Plates
- from Andy Wang
- Preparation of Gamete Autolysin
- discussion from bionet.chlamydomonas, several contributors
- Preparation of Gamete Autolysin
- improved protocol, from Sabine Waffenschmidt May 2006
- Chlamydomonas Cells Sticking to Glass
- compiled by Tjibbe-Chris Kuipers for bionet.chlamydomonas; several contributors
- Tris as N Source for Chlamydomonas?
- compiled by Xavier Reboud for bionet.chlamydomonas; several contributors
- Fixation Method for Mitotic Cells
- from Peter Luykx
- Minipreps of DNA from Chlamydomonas Cultures
- compiled by Hank Latorella for bionet.chlamydomonas; several contributors
- Transformation of Chlamydomonas with Glass Beads
- from Karen Kindle
- Improving Transformation Efficiencies
- compiled by Roel Funke for bionet.chlamydomonas; several contributors
- Problems Cutting Genomic DNA
- from Cliff Zeyl
- Antisense and Gene Expression
- from Karen Kindle
- PCR of Chlamydomonas Nuclear DNA
- from Barbara Randolph-Anderson
- Extraction of RNA from Chlamydomonas
- from Takako Kato
- Sequencing GC-Rich Nuclear DNA from Chlamydomonas
- compiled by Emma Berta Gutierrez for bionet.chlamydomonas; several contributors
- Transposon Insertions
- compiled by Kris Niyogi for bionet.chlamydomonas; several contributors
- Encouraging Stubborn Cells to Grow
- from the Chlamy Center
- My Cells Don’t Swim/Lack Flagella/Grow in Clumps
- from Pete Lefebvre, Chlamy Center
- Craig’s Chlamy Corner
- This page by Craig Amundsen includes methods protocols used in the Lefebvre/Silflow laboratory at the University of Minnesota. These files were deleted by an overzealous administrator at that university, but fortunately were archived on the Wayback Machine. Some obsolete links have been removed, and we were unable to retrieve some image files, but the methods files were recovered.