The use of whole genome sequencing to identify mutant lesions
Lin H, Miller ML, Granas DM, Dutcher SK. Whole genome sequencing identifies a deletion in protein phosphatase 2A that affects its stability and localization inChlamydomonas reinhardtii. PLoS Genet. 2013;9(9):e1003841. doi: 10.1371/journal.pgen.1003841. Epub 2013 Sep 26. PubMed PMID: 24086163; PubMed Central PMCID: PMC3784568.
Research positions in microalgal cell biology
Temporary Full Time Technician, Scientist and Intern Research positions in microalgal cell biology: Full time (1-2 years) for a research technician, scientist and intern in a research project focusing on protein expression in microalgae algae at North Carolina Central University located at the North Carolina Research Campus in Kannapolis (20 minutes from Charlotte). Job Description: Primary … Continued
17th International Meeting on the Cell and Molecular Biology of Chlamydomonas
From Hideya Fukuzawa: The next Chlamydomonas meeting will be held at the Kyoto International Conference Center, Takaragaike, Sakyo-ku, Kyoto 606-0001 Japan, on June 26-July 1, 2016. The plenary session will start on Sunday, June 26, in late afternoon. July 1st is just for departure.
Chlamy mutant library, update
Dear Chlamy community, We have set up another opportunity for labs who haven’t yet received a mutant from the pilot collection to obtain one [see previous announcement below, posted in December]. If you are interested, please see the website below and use the form at the bottom to submit a request by June 23rd, 2014. … Continued
New link for Chlamydomonas at Phytozome
Verson 5.5 of the C. reinhardtii genome is now available at http://phytozome.jgi.doe.gov/pz/portal.html#!info?alias=Org_Creinhardtii
Batch conversion of Chlamydomonas gene identifiers
The tool for converting batches of Chlamydomonas gene identifiers between different annotation versions has been updated to include all versions from JGI v3 onwards. The tool is available here: http://pathways.mcdb.ucla.edu/algal/id_conversion.html The versions that can be converted are JGI v3, v4, v4.3 (Augustus u10.2), v5.3.1, v5.5 as well as Augustus u5 and u9 and GenBank accessions … Continued
16th International Conference on the Cell and Molecular Biology of Chlamydomonas
Asilomar Conference Grounds, Pacific Grove CA, June 8-14, 2014 Organizers: Krishna Niyogi and Winfield Sale
Chlamy mutant library
Dear Chlamy community, Thanks to letters of support from many of you, the Chlamy mutant library project is now well underway, and we currently have a pilot collection covering ~10% of all genes. While the paper is still a ways away, we have received many requests for collaborative distribution of individual mutants before we publish. … Continued
BAC library is OK
From Olivier Vallon, IBPC, Paris: Recently, concerns were raised about possible plate swaps in the BAC library distributed by Clemson University Genomics Institute and sequenced by JGI under the accessions PTQ…. While the copy #23 received by the Paris group indeed suffers from plate swaps, this seems to be due to a one-time error of … Continued
BAC library debugged
From Katia Wostrikoff and Olivier Vallon, IBPC, Paris: While trying to complement mutations in specific regions of the genome using the BAC library from the Silflow/Lefebvre labs, we have come to realize that for many plates, the sequences indicated by the JGI browser (http://genome.jgi-psf.org/Chlre4/Chlre4.home.html) were not correct. We have systematically sequenced BAC ends from all … Continued