CC-215 sr-u-sm2 act1 mt-
$30.00
From Ruth Sager, Hunter College, April 1974
Phenotype: antibiotic resistant (cycloheximide, streptomycin)
This is one of the strains used by Sager and Ramanis in their series of papers on mapping chloroplast genes by pedigree and cosegregation analysis. It combines the chloroplast marker sr-u-sm2 (in the rps12 gene; see Liu et al. 1989) with the nuclear marker act1, which permitted identification of second-division segregation events in meiosis.
Phenotypic tests at Duke showed that CC-215 carries a nuclear neamine resistance marker, presumably nr1 although allelism tests have not been done to confirm this. The cycloheximide resistance marker was shown to be act1 in a cross to ac12.
Like most of Sager’s strains, CC-215 grows on nitrate as sole N source. This property is in contrast to most strains from the Ebersold/Levine background, which carry the nit1 and nit2 mutations.
Liu XQ, Gillham NW, Boynton JE (1989) Chloroplast ribosomal protein gene rps12 of Chlamydomonas reinhardtii. Wild-type sequence, mutation to streptomycin resistance and dependence, and function in Escherichia coli. J Biol Chem 264:16100-16108