CC-2530 vfl2 mt+
$30.00
From Jonathan Jarvik, Carnegie-Mellon University, October 1990
Phenotype: variable number of flagella
This mutant has a variable number of flagella, usually zero to six. The flagella are of normal length, and the cells appear to be of normal size and shape. The mutation was UV-induced in strain ‘NO mt+’ (equivalent to CC-620) by Kuchka and Jarvik, originally vfl-2-220. Taillon et al. determined that vfl2 is a G to A transition mutation in the structural gene for centrin, producing a Glu to Lys change at position 101, the first amino acid of the E-helix of the protein’s third EF-hand.
Kuchka MR, Jarvik JW (1982) Analysis of flagellar size control using a mutant of Chlamydomonas reinhardtii with a variable number of flagella. J Cell Biol 92:170-175
Jarvik JW, Suhan JP (1991) The role of the flagellar transition region: inferences from the analysis of aChlamydomonas mutant with defective transition region structures. J Cell Sci 99:731-740
Taillon BE, Adler SA, Suhan JP, Jarvik JW (1992) Mutational analysis of centrin: an EF-hand protein associated with three distinct contractile fibers in the basal body apparatus of Chlamydomonas. J Cell Biol 119:1613-1624
Harper JDI (1999) Chlamydomonas Cell Cycle Mutants. Int Rev Cytol 189:131-176