CC-4924 rbcL∆/rbcS1-LSRR mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, October 2014
Phenotype: requires elevated CO2 for photosynthetic growth, zeocin and spectinomycin resistant
This mutant was created by Fuqiao Xu in Spreitzer’s group by nuclear transformation of rbcLΔ/rbcSΔ-1A mt- (CC-4697) with a plasmid (pSS1-LSRR) that has the Chlamydomonas Rubisco small-subunit coding region of pSS1-ITP (Genkov et al. 2010) replaced with the codon-optimized sequence of Rhodospirillum rubrum Rubisco (Somerville and Somerville 1984). The mutant grows very slowly on minimal medium only when provided with 5% CO2 in air. It expresses only a low level of R. rubrum Rubisco. The strain has been maintained with acetate medium in darkness since its recovery in 2012.
Genkov T, Meyer M, Griffiths H, Spreitzer RJ (2010) Functional hybrid Rubisco enzymes with plant small subunits and algal large subunits: Engineered rbcS cDNA for expression in Chlamydomonas. J Biol Chem 285:19833-19841
Somerville CR, Somerville SC (1984) Cloning and expression of the Rhodospirillum rubrum ribulosebisphosphate carboxylase gene in E. coli. Molec Gen Genet 193:214-219
Out of stock