CC-5604 amc9; NUO5(K230R)-FLAG mt- [transformant #7]
$30.00
From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020
Genotype: mt-; arg7-8; nuo5::APHVII; NUO5K230R-FLAG; APHVIII
This is the amc9 strain complemented with the NUO5 gene containing the K230R substitution with a C-terminal FLAG tag. It was created by mutagenesis of amc9 with plasmid pRS426 containing the NUO5 gene encoding the K230R substitution with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.
It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine.
Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.
Out of stock