P-228 cpDNA BamHI-HindIII (4.8 kb) spr-u-1-6-2

$30.00

Boynton-Gillham laboratory, Duke University, 1989

Chloroplast DNA Bam-HindIII (4.8 kb) in pUC18; 16S through 5′ part of 23S rRNA gene; from CC-110, contains spectinomycin resistance mutation spr-u-1-6-2; see P-667 for the same insert in an XL1 blue strain

host strain: JM83
amp resistant

 

More information

Strain: CC-110 spr-u-1-6-2 mt+

Insert: BamHI-HindIII (4.8 kb) from BamHI 11/12

Genes present: 16S rRNA, 23Ss rRNA (5′ end)

Vector: pUC18

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Scott Newman in Boynton/Gillham lab, 1989
P-228

The spr-u-1-6-2 mutations is an A-> G change at bp 1123 in the C. reinhardtii 16S rRNA gene which causes loss of an AatII restriction site and confers high level spectinomycin resistance. See Harris et al., Genetics 123, 281-292 (1989). Used in rRNA transformation experiments by Newman et al., Genetics 126, 875-888 (1990).