P-260 cpDNA KpnI (3.1 kb) DCMU4

$30.00

Boynton-Gillham laboratory, Duke University, 1990

Chloroplast DNA KpnI (3.1 kb) in BSKS+; contains psbA exons 4 and 5 from DCMU4 mutant, CC-2473

host strain: JM109
amp resistant

 

More information

Strain: CC-2473 DCMU4

Insert: KpnI (3.1 kb)

Genes present: psbA exons 4 and 5

Vector: BSKS+

Bacterial host strain: JM109

Selectable marker: amp-r, white on X-gal

Origin: Amnon Lers in Boynton/Gillham lab, 1990

P-260

DCMU4 is a ser->ala mutation at aa 264 in the C. reinhardtii psbA 5th exon, conferring resistance to DCMU and atrazine. The insert was subcloned into the KS+ KpnI site in both orientations. In P-260A, the psbA direction is from the KS SacI site towards the KpnI site; in P-260B, it is from the KS KpnI site towards the SacI site.