* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $1000.00. Subscriptions cannot be used for CLiP mutants, BACs nor fosmids.

pCM0_TB129

Name: pCM0_TB129
SKU: M-132 (spec)
Price: 30.00 USD
Availability: InStock

$30.00

From Thomas Baier, Olaf Kruse lab, Bielefeld University, August 2021

Host strain: DH5α
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): A1_B2
Brick name: P_AßSAP(i)(Pro + 5’UTR)
Brick type: promoter + 5’UTR
Brick function: Synthetic HSP70A/ßtub promoter, which drives 4xstronger expression compared to pAR when transgene is expressed to the cytosol
Source of Brick: Einhaus et al. 2021
Acceptor plasmid: pICH41295
Designer / Builder: A. Einhaus and T. Baier

Sequence

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pCM0_TB129

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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