Core
$250.00
C. reinhardtii CC-1690, Lambda Zap II
Library prepared by John Davies and Jeffrey McDermott. cDNA was prepared from CC-1690 cells grown to mid-log phase in TAP (acetate-containing) medium in the light, TAP medium in the dark, HS (minimal) medium in ambient levels of CO2 and HS medium bubbled with 5% CO2.
ESTs from this library are identified with the project IDs 874, 894 and 1024.
Stress I
$250.00
C. reinhardtii CC-1690, Lambda Zap II
Library prepared by John Davies and Jeffrey McDermott. cDNA was prepared from CC-1690 cells grown to mid-log phase in TAP-N (30 min, 1hr, 4hr), TAP-S (30 min, 1hr, 4hr), TAP-P (4hr, 12hr, 24hr), NO3 to NH4 (30min, 1hr, 4hr) and NH4 to NO3 (30min, 1hr, 4hr).
ESTs from this library are identified with the project ID 963.
Stress II
$250.00
C. reinhardtii CC-1690, Lambda Zap II
Library prepared by John Davies and Jeffrey McDermott. cDNA was prepared from CC-1690 cells grown to mid-log phase in TAP (NH4+ – containing) and shifted to TAP – NO3- (24hrs); H2 production conditions (0, 12hr, 24hr) see Melis et al.,(2000) Plant Phys. 122: 127-135; TAP + H2O2 (1, 12, 24 hr); TAP + sorbitol (1, 2, 6, 24 hr); TAP + Cd (1, 2, 6, 24 hr).
ESTs from this library are identified with the project ID 1031.
S1D2
$250.00
C. reinhardtii CC-2290, Lambda Zap II
Library prepared by John Davies and Jeffrey McDermott. cDNA was prepared from strain CC-2290 (Minnesota isolate of C. reinhardtii) grown to mid-log phase in TAP (acetate containing) medium in the light.
ESTs from this library are identified with the project ID 925.
Deflagellation
$250.00
Library prepared by John Davies and Jeffrey McDermott. cDNA was prepared from CC-1690 cells which had been re-synthesizing flagella for 15, 30 and 60 min after being deflagellated by pH shock. PolyA mRNA was purified from each sample, pooled and cDNA synthesized.
ESTs from this library are identified with the project ID 1030.
Gamete and Zygote
$250.00
Library prepared by Hui Zhao, Min Lu, Jeffrey McDermott, William J. Snell and John Davies. cDNA was prepared in the laboratory of William Snell from strain 21gr mt+ and from a mt- strain (Sager 6145, CC-1691) which had been transfered to nitrogen-deficient medium and sampled at 3, 8, 10, 12, 15, and 17 hours during the period of gametogenesis and at 30 and 60 minutes following mixing of the two mating types. PolyA mRNA was purified from each sample, pooled and cDNA synthesized.
ESTs from this library are identified with the project ID 1112.
-S
$250.00
-Sulfur
+-S/-Fe
$250.00
+-S/-Fe