CLiP Strains

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) UMM7 (Cre08.g380000), chromosome_8 base 4164193 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • UMM7
  • Chromosome:
  • 8

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre01.g023650, chromosome_1 base 3678814 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre01.g023650
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre01.g016570, chromosome_1 base 2803550 (genome v5.5), intron – position has 95% probability of being correct
2) Cre01.g016570, chromosome_1 base 2803606 (genome v5.5), intron – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre01.g016570
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) CAV5 (Cre17.g702850), chromosome_17 base 926523 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • CAV5
  • Chromosome:
  • 17

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) CGL112 (Cre13.g566200), chromosome_13 base 666784 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • CGL112
  • Chromosome:
  • 13

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre01.g046650, chromosome_1 base 6354193 (genome v5.5), intron – WARNING position has 58% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre01.g046650
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) OPR39,RAT2 (Cre09.g388372), chromosome_9 base 2885671 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • OPR39,RAT2
  • Chromosome:
  • 9

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre12.g495951, chromosome_12 base 822658 (genome v5.5), 3’UTR – position has 95% probability of being correct
2) Cre12.g495951, chromosome_12 base 822676 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre12.g495951
  • Chromosome:
  • 12

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) RSE1,RSEP1 (Cre17.g730100), chromosome_17 base 4192231 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • RSE1,RSEP1
  • Chromosome:
  • 17

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre06.g293300, chromosome_6 base 6528206 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre06.g293300
  • Chromosome:
  • 6

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre09.g397450, chromosome_9 base 1595667 (genome v5.5), CDS – WARNING position has 58% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre09.g397450
  • Chromosome:
  • 9

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre01.g023400, chromosome_1 base 3633786 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre01.g023400
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Carnegie Institution for Science, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre13.g588650, chromosome_13 base 3640284 (genome version 5.5), CDS

The listed mapped insertions have a ~25% chance of being incorrect, and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre13.g588650
  • Chromosome:
  • 13

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) FAP48,RYR1 (Cre16.g665450), chromosome_16 base 3104623 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • FAP48,RYR1
  • Chromosome:
  • 16

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre12.g493300, chromosome_12 base 486904 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre12.g493300, chromosome_12 base 486906 (genome v5.5), CDS – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre12.g493300
  • Chromosome:
  • 12

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) FAP163 (Cre10.g428664), chromosome_10 base 1458454 (genome v5.5), gene_edge/mRNA_edge/3’UTR – position has 95% probability of being correct
2) FAP163 (Cre10.g428664), chromosome_10 base 1458462 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • FAP163
  • Chromosome:
  • 10

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) NAT31 (Cre13.g586450), chromosome_13 base 3310926 (genome v5.5), 5’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • NAT31
  • Chromosome:
  • 13

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre01.g009765, chromosome_1 base 1817197 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre01.g009765, chromosome_1 base 1817252 (genome v5.5), CDS – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre01.g009765
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) PGP1 (Cre03.g168700), chromosome_3 base 3636481 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • PGP1
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre02.g110700, chromosome_2 base 5774123 (genome v5.5), CDS – WARNING position has 73% probability of being correct
2) Cre10.g422250, chromosome_10 base 675733 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g110700, Cre10.g422250
  • Chromosome:
  • 10, 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) MAPKKK4 (Cre01.g030600), chromosome_1 base 4495228 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • MAPKKK4
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre19.g750797, scaffold_19 base 127588 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre19.g750797
  • Chromosome:
  • 19

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) CAL3,MOT15 (Cre02.g112300), chromosome_2 base 6008753 (genome v5.5), CDS – position has 95% probability of being correct
2) CAL3,MOT15 (Cre02.g112300), chromosome_2 base 6008755 (genome v5.5), CDS – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • CAL3,MOT15
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) TRXy,TRX6 (Cre10.g446100), chromosome_10 base 3700760 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • TRXy,TRX6
  • Chromosome:
  • 10

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre11.g477700, chromosome_11 base 2858614 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre11.g477700
  • Chromosome:
  • 11

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Carnegie Institution for Science, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre11.g476026, chromosome_11 base 2530347 (genome version 5.5), intron

The listed mapped insertions have a ~25% chance of being incorrect, and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre11.g476026
  • Chromosome:
  • 11

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) SRX1 (Cre17.g729950), chromosome_17 base 4169157 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • SRX1
  • Chromosome:
  • 17

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre06.g293100, chromosome_6 base 6499922 (genome v5.5), 5’UTR – position has 95% probability of being correct
2) intergenic, chromosome_6 base 6499953 (genome v5.5) – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre06.g293100, intergenic
  • Chromosome:
  • 6

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) Cre01.g036100, chromosome_1 base 5191484 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct
2) Cre06.g278157, chromosome_6 base 3761382 (genome v5.5), 5’UTR – position has 95% probability of being correct
3) Cre06.g278157, chromosome_6 base 3761400 (genome v5.5), 5’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre01.g036100, Cre06.g278157
  • Chromosome:
  • 1, 6

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre04.g228650, chromosome_4 base 3619166 (genome v5.5), intron – position has 95% probability of being correct
2) Cre04.g228650, chromosome_4 base 3619317 (genome v5.5), intron – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre04.g228650
  • Chromosome:
  • 4