CLiP Strains

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre06.g278204, chromosome_6 base 4021314 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre06.g278204
  • Chromosome:
  • 6

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre03.g189700, chromosome_3 base 5880591 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre03.g189700
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre14.g621050, chromosome_14 base 1934319 (genome v5.5), 3’UTR – position has 95% probability of being correct
2) Cre14.g621050, chromosome_14 base 1934337 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre14.g621050
  • Chromosome:
  • 14

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre07.g325745, chromosome_7 base 1923873 (genome v5.5), intron – position has 95% probability of being correct
2) Cre07.g325745, chromosome_7 base 1923879 (genome v5.5), intron – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre07.g325745
  • Chromosome:
  • 7

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre02.g100950, chromosome_2 base 4599051 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g100950
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre11.g467687, chromosome_11 base 1160722 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre11.g467687
  • Chromosome:
  • 11

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) URN2 (Cre04.g224100), chromosome_4 base 2814095 (genome v5.5), 5’UTR – position has 95% probability of being correct
2) URN2 (Cre04.g224100), chromosome_4 base 2814107 (genome v5.5), 5’UTR – position has 95% probability of being correct
3) URN2 (Cre04.g224100), chromosome_4 base 2814107 (genome v5.5), 5’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • URN2
  • Chromosome:
  • 4

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 4 mapped insertions:
1) Cre07.g315850, chromosome_7 base 493609 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre07.g315850, chromosome_7 base 493619 (genome v5.5), CDS – position has 95% probability of being correct
3) CYG5 (Cre10.g431250), chromosome_10 base 1843368 (genome v5.5), 3’UTR – position has 95% probability of being correct
4) CYG5 (Cre10.g431250), chromosome_10 base 1843389 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • CYG5, Cre07.g315850
  • Chromosome:
  • 10, 7

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre02.g118200, chromosome_2 base 6726762 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g118200
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre06.g278226, chromosome_6 base 4137588 (genome v5.5), 3’UTR – position has 95% probability of being correct
2) Cre06.g278226, chromosome_6 base 4137588 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre06.g278226
  • Chromosome:
  • 6

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre02.g079200, chromosome_2 base 872492 (genome v5.5), intron – WARNING position has 73% probability of being correct
2) Cre17.g745597, chromosome_17 base 6878097 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g079200, Cre17.g745597
  • Chromosome:
  • 17, 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 4 mapped insertions:
1) PDE7 (Cre09.g389875), chromosome_9 base 3537122 (genome v5.5), 3’UTR – position has 95% probability of being correct
2) PDE7 (Cre09.g389875), chromosome_9 base 3537129 (genome v5.5), 3’UTR – position has 95% probability of being correct
3) EEF1A2 (Cre09.g408250), chromosome_9 base 6581751 (genome v5.5), 3’UTR – position has 95% probability of being correct
4) EEF1A2 (Cre09.g408250), chromosome_9 base 6581766 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • EEF1A2, PDE7
  • Chromosome:
  • 9

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) OPR76,RAP12,CLR10 (Cre15.g638401), chromosome_15 base 1071980 (genome v5.5), 5’UTR – WARNING position has 58% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • OPR76,RAP12,CLR10
  • Chromosome:
  • 15

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre07.g313100, chromosome_7 base 129856 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct
2) intergenic, chromosome_9 base 801771 (genome v5.5) – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre07.g313100, intergenic
  • Chromosome:
  • 7, 9

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre02.g143858, chromosome_2 base 7556201 (genome v5.5), 3’UTR – position has 95% probability of being correct
2) Cre02.g143858, chromosome_2 base 7556209 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g143858
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre08.g382250, chromosome_8 base 4446195 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre08.g382250, chromosome_8 base 4446199 (genome v5.5), CDS – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre08.g382250
  • Chromosome:
  • 8

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre06.g283300, chromosome_6 base 5331261 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre06.g283300
  • Chromosome:
  • 6

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre07.g338200, chromosome_7 base 3766710 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre07.g338200
  • Chromosome:
  • 7

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) Cre07.g353400, chromosome_7 base 5820007 (genome v5.5), CDS – WARNING position has 73% probability of being correct
2) Cre07.g353400, chromosome_7 base 5821345 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct
3) Cre13.g589450, chromosome_13 base 3743115 (genome v5.5), intron – WARNING position has 58% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre07.g353400, Cre13.g589450
  • Chromosome:
  • 13, 7

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre14.g618550, chromosome_14 base 1525927 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre14.g618550
  • Chromosome:
  • 14

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre10.g444600, chromosome_10 base 3433536 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre10.g444600
  • Chromosome:
  • 10

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) GPX3 (Cre03.g197750), chromosome_3 base 6721982 (genome v5.5), CDS – position has 95% probability of being correct
2) GPX3 (Cre03.g197750), chromosome_3 base 6722016 (genome v5.5), intron – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • GPX3
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) CLC1 (Cre03.g155650), chromosome_3 base 1953560 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct
2) VAMP74,VAM4 (Cre04.g224800), chromosome_4 base 2956410 (genome v5.5), CDS – WARNING position has 73% probability of being correct
3) VAMP74,VAM4 (Cre04.g224800), chromosome_4 base 2957365 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • CLC1, VAMP74,VAM4
  • Chromosome:
  • 3, 4

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) PTK7 (Cre03.g158300), chromosome_3 base 2314707 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • PTK7
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) SRH5 (Cre03.g162701), chromosome_3 base 2859170 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • SRH5
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) intergenic, chromosome_2 base 6285459 (genome v5.5) – WARNING position has 73% probability of being correct
2) TTL2,MOT11 (Cre02.g117350), chromosome_2 base 6608733 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • TTL2,MOT11, intergenic
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre02.g117600, chromosome_2 base 6661030 (genome v5.5), intron – position has 95% probability of being correct
2) Cre02.g117600, chromosome_2 base 6661056 (genome v5.5), intron – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g117600
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) ADH8 (Cre03.g207550), chromosome_3 base 7247416 (genome v5.5), intron – WARNING position has 58% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • ADH8
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre04.g228000, chromosome_4 base 3503996 (genome v5.5), intron – WARNING position has 58% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre04.g228000
  • Chromosome:
  • 4

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre02.g095090, chromosome_2 base 3328912 (genome v5.5), 3’UTR – position has 95% probability of being correct
2) Cre02.g095090, chromosome_2 base 3328914 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g095090
  • Chromosome:
  • 2