CLiP Strains

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre01.g052750, chromosome_1 base 7324912 (genome v5.5), intron – position has 95% probability of being correct
2) Cre01.g052750, chromosome_1 base 7324915 (genome v5.5), intron – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre01.g052750
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) intergenic, chromosome_6 base 7112387 (genome v5.5) – WARNING position has 73% probability of being correct
2) Cre08.g369150, chromosome_8 base 2139142 (genome v5.5), intron – WARNING position has 73% probability of being correct
3) CNK6 (Cre12.g514650), chromosome_12 base 3707850 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • CNK6, Cre08.g369150, intergenic
  • Chromosome:
  • 12, 6, 8

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) PQQ2 (Cre01.g017600), chromosome_1 base 2896220 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • PQQ2
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) FAP95 (Cre13.g573500), chromosome_13 base 1619108 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • FAP95
  • Chromosome:
  • 13

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) Cre02.g100950, chromosome_2 base 4601238 (genome v5.5), intron – WARNING position has 58% probability of being correct
2) CHI7 (Cre13.g570700), chromosome_13 base 1269777 (genome v5.5), intron – WARNING position has 73% probability of being correct
3) Cre14.g614050, chromosome_14 base 981289 (genome v5.5), intron – WARNING position has 58% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • CHI7, Cre02.g100950, Cre14.g614050
  • Chromosome:
  • 13, 14, 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre03.g200100, chromosome_3 base 8199924 (genome v5.5), 3’UTR – position has 95% probability of being correct
2) Cre03.g200100, chromosome_3 base 8199929 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre03.g200100
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre08.g375801, chromosome_8 base 3593309 (genome v5.5), 3’UTR – WARNING position has 58% probability of being correct
2) BLZ18 (Cre13.g568350), chromosome_13 base 983155 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • BLZ18, Cre08.g375801
  • Chromosome:
  • 13, 8

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre01.g027050, chromosome_1 base 4116650 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre01.g027050
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre03.g210065, chromosome_3 base 9028359 (genome v5.5), CDS – WARNING position has 58% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre03.g210065
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) SSD3 (Cre12.g550900), chromosome_12 base 8168215 (genome v5.5), 5’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • SSD3
  • Chromosome:
  • 12

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre09.g396050, chromosome_9 base 1821191 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre09.g396050
  • Chromosome:
  • 9

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre02.g074370, chromosome_2 base 163740 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g074370
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) APE2,TPT3 (Cre01.g045550), chromosome_1 base 6390321 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • APE2,TPT3
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) Cre06.g278400, chromosome_6 base 4835499 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct
2) Cre17.g745297, chromosome_17 base 6841352 (genome v5.5), CDS – position has 95% probability of being correct
3) Cre17.g745297, chromosome_17 base 6841362 (genome v5.5), CDS – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre06.g278400, Cre17.g745297
  • Chromosome:
  • 17, 6

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre03.g155500, chromosome_3 base 1941009 (genome v5.5), intron – position has 95% probability of being correct
2) Cre03.g155500, chromosome_3 base 1941071 (genome v5.5), intron – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre03.g155500
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) HDA10 (Cre11.g477800), chromosome_11 base 2881768 (genome v5.5), 3’UTR – position has 95% probability of being correct
2) HDA10 (Cre11.g477800), chromosome_11 base 2881768 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • HDA10
  • Chromosome:
  • 11

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre11.g467651, chromosome_11 base 866008 (genome v5.5), 5’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre11.g467651
  • Chromosome:
  • 11

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre06.g283500, chromosome_6 base 5347049 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre06.g283500, chromosome_6 base 5347049 (genome v5.5), CDS – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre06.g283500
  • Chromosome:
  • 6

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre02.g089500, chromosome_2 base 2169434 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre02.g089500, chromosome_2 base 2169437 (genome v5.5), CDS – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g089500
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre07.g314250, chromosome_7 base 299541 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre07.g314250
  • Chromosome:
  • 7

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre13.g565900, chromosome_13 base 635412 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre13.g565900, chromosome_13 base 635414 (genome v5.5), CDS – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre13.g565900
  • Chromosome:
  • 13

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) Cre02.g094900, chromosome_2 base 2888904 (genome v5.5), intron – WARNING position has 73% probability of being correct
2) Cre09.g410332, chromosome_9 base 6927145 (genome v5.5), 3’UTR – position has 95% probability of being correct
3) Cre09.g410332, chromosome_9 base 6927170 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g094900, Cre09.g410332
  • Chromosome:
  • 2, 9

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre03.g150800, chromosome_3 base 1380436 (genome v5.5), intron – WARNING position has 58% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre03.g150800
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre12.g507051, chromosome_12 base 2425085 (genome v5.5), intron – position has 95% probability of being correct
2) Cre12.g507051, chromosome_12 base 2425086 (genome v5.5), intron – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre12.g507051
  • Chromosome:
  • 12

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) ELG29 (Cre09.g388450), chromosome_9 base 2877004 (genome v5.5), intron – position has 95% probability of being correct
2) ELG29 (Cre09.g388450), chromosome_9 base 2877020 (genome v5.5), intron – position has 95% probability of being correct
3) Cre16.g675200, chromosome_16 base 6195400 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre16.g675200, ELG29
  • Chromosome:
  • 16, 9

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) intergenic, chromosome_7 base 3987333 (genome v5.5) – WARNING position has 73% probability of being correct
2) Cre17.g697900, chromosome_17 base 237283 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre17.g697900, intergenic
  • Chromosome:
  • 17, 7

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 4 mapped insertions:
1) Cre02.g079150, chromosome_2 base 868685 (genome v5.5), intron – WARNING position has 58% probability of being correct
2) Cre02.g079150, chromosome_2 base 868825 (genome v5.5), CDS – WARNING position has 73% probability of being correct
3) DHP1 (Cre02.g097000), chromosome_2 base 4011398 (genome v5.5), 3’UTR – WARNING position has 58% probability of being correct
4) DHP1 (Cre02.g097000), chromosome_2 base 4011404 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre02.g079150, DHP1
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) NAT6 (Cre01.g009550), chromosome_1 base 1777046 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct
2) LCI28 (Cre09.g394658), chromosome_9 base 4286655 (genome v5.5), CDS – position has 95% probability of being correct
3) LCI28 (Cre09.g394658), chromosome_9 base 4286658 (genome v5.5), CDS – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • LCI28, NAT6
  • Chromosome:
  • 1, 9

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre09.g394102, chromosome_9 base 4190347 (genome v5.5), 3’UTR – position has 95% probability of being correct
2) Cre09.g394102, chromosome_9 base 4190347 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • Cre09.g394102
  • Chromosome:
  • 9

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) HLD2 (Cre01.g010550), chromosome_1 base 1957107 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.

  • Locus:
  • HLD2
  • Chromosome:
  • 1