MoClo Level 0 Parts
pCM0-110
$30.00
$30.00
From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B5
Brick name: CrMSPTS1 (C-ter, CDS)
Brick type: Signal and Targeting peptides (CDS)
Brick function: Allows the targetting to the microbodies
Source of Brick: Lauersen et al, 2015
Acceptor plasmid: pAGM1301
Designer / Builder: KJ. Lauersen
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
pCM0-111
$30.00
$30.00
From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B5
Brick name: ER ret (C-ter, CDS)
Brick type: Signal and Targeting peptides (CDS)
Brick function: Allows retention in Endoplasmic Reticulum
Source of Brick: pBR30 (Rasala et al, 2013)
Acceptor plasmid: pAGM1301
Designer / Builder: P. Auroy
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
pCM0-112
$30.00
$30.00
From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B5
Brick name: mRuby2 (i2)-Strep (C-ter, CDS)
Brick type: Reporter gene (CDS)
Brick function: Add an mRuby2 (with RBCS2 intron 2) + StrepII tag in C-terminal
Source of Brick: Lauersen et al, 2015
Acceptor plasmid: pAGM1301
Designer / Builder: KJ. Lauersen
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
pCM0-113
$30.00
$30.00
From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B5
Brick name: mVenus (i2)-Strep (C-ter, CDS)
Brick type: Reporter gene (CDS)
Brick function: Add an mVenus (with RBCS2 intron 2) + StrepII tag in C-terminal
Source of Brick: Lauersen et al, 2015
Acceptor plasmid: pAGM1301
Designer / Builder: KJ. Lauersen
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
pCM0-114
$30.00
$30.00
From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B6-C1
Brick name: TPSAD (3´UTR + Ter)
Brick type: 3’UTR + Terminator
Brick function: 3’UTR and Terminator for expression
Source of Brick: Cre05.g238332
Acceptor plasmid: pICH41276
Designer / Builder: P. Crozet
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
pCM0-115
$30.00
$30.00
From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B6-C1
Brick name: TRBCS2 (3´UTR + Ter)
Brick type: 3’UTR + Terminator
Brick function: 3’UTR and Terminator for expression
Source of Brick: Cre02.g120150
Acceptor plasmid: pICH41276
Designer / Builder: P. Crozet
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
pCM0-116
$30.00
$30.00
From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B6-C1
Brick name: TRPS29 (3´UTR + Ter)
Brick type: 3’UTR + Terminator
Brick function: 3’UTR and Terminator for expression
Source of Brick: Lauersen et al, 2015
Acceptor plasmid: pICH41276
Designer / Builder: D. Gangl
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
pCM0-117
$30.00
$30.00
From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B6-C1
Brick name: TCA1 (3´UTR + Ter)
Brick type: 3’UTR + Terminator
Brick function: 3’UTR and Terminator for expression
Source of Brick: Lauersen et al, 2015
Acceptor plasmid: pICH41276
Designer / Builder: P. Mehrshahi
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
pCM0-118
$30.00
$30.00
From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B6-C1
Brick name: TβTUB2 (3’UTR + Ter)
Brick type: 3’UTR + Terminator
Brick function: 3’UTR and Terminator for strong expression
Source of Brick: Cre12.g549550
Acceptor plasmid: pICH41276
Designer / Builder: L. Westrich
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
pCM0-119
$30.00
$30.00
From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B6-C1
Brick name: TRPL23 (i) (3´UTR + Ter)
Brick type: 3’UTR + Terminator
Brick function: 3’UTR (with intron) and Terminator for expression
Source of Brick: Cre04.g211800
Acceptor plasmid: pICH41276
Designer / Builder: B. Spaniol
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
pCM0-120
$30.00
$30.00
From Félix de Carpentier, Institute of Physico-Chemical Biology-Paris, February 2020
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018) and de Carpentier et al. (2020).
Host strain: NEB® 10-beta
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B3-B5
Brick name: BSR (CDS)
Brick type: Antibiotic Resistance gene (CDS)
Brick function: Resistance to Blasticidin in Chlamy
Source of Brick: Synthetized
Acceptor plasmid: pICH41308
Designer / Builder: F. de Carpentier and J. Le Peillet
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
de Carpentier et al. (2020) Blasticidin S deaminase: a new efficient selectable marker for Chlamydomonas reinhardtii. Frontiers in Plant Science 11:242
de Carpentier F, Le Peillet J, Boisset ND, Crozet P, Lemaire SD, Danon A. Blasticidin S Deaminase: A New Efficient Selectable Marker for Chlamydomonas reinhardtii. Front Plant Sci. 2020 Mar 5;11:242. doi: 10.3389/fpls.2020.00242. PMID: 32211000; PMCID: PMC7066984.
pCM0-TB_004
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: DH5a
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B6-C1
Brick name: FDX (3´UTR + Ter)
Brick type: 3’UTR + Terminator
Brick function: 3’UTR and Terminator for expression (Lopez-Paz et al. 2017)
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41276
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
López-Paz C, Liu D, Geng S, Umen JG. Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression. Plant J. 2017 Dec;92(6):1232-1244. doi: 10.1111/tpj.13731. Epub 2017 Nov 18. PMID: 28980350; PMCID: PMC5718938.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
Einhaus A, Baier T, Rosenstengel M, Freudenberg RA, Kruse O. Rational Promoter Engineering Enables Robust Terpene Production in Microalgae. ACS Synth Biol. 2021 Apr 16;10(4):847-856. doi: 10.1021/acssynbio.0c00632. Epub 2021 Mar 25. PMID: 33764741.
pCM0-TB_009
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: DH5a
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B5
Brick name: StrepII (i2) (C-ter, CDS)
Brick type: Immuno- and purification tag
Brick function: Add a StrepII tag (with RBCS2 intron 2) + STOP in C-terminal
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pAGM1301
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB_028
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: DH5a
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B4
Brick name: mCerulean3 (2xi1) (CDS)
Brick type: Reporter gene (CDS)
Brick function: mCerulean3 reporter (with 2 copies of the RBCS2 intron 1) + GSG-Linkers in B4
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pAGM1299
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0-TB288
$30.00
$30.00
From Nick Jacobebbinghaus and Dr. Thomas Baier, Bielefeld University, Germany, April 2024
Host strain: DH5a
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al.): B5
Brick name: pCM0-TB288
Brick type: Antibiotic Resistance gene (CDS)
Brick function: A synthetic construct, which allows bicistronic co-expression from a joint mRNA encoding an upstream-located GOI and the downstream-located selection marker aphVIII. A variable ORF 1 (B3 and B4 position) will be terminated by addition of a GS-Linker (8 aa long), the RBCS intron 2 and a C-terminal FLAG-tag including a Stop-codon. A 249 bp long, native intergenic sequence from the C. reinhardtii nuclear genome (gene locus Cre01.g022450/Cre01.g022462) allows transcriptional read through and ribosome assembly for individual translation of downstream aphVIII. A suitable terminator including a 3’UTR is required (in B6_C1 position) and terminates transcription. This construct transfers the aphVIII selection pressure on a joint mRNA for GOI expression, allows bicistronic expression designs and simplifies screening procedure after nuclear transformations due to increased expression level of the upstream-located GOI. Plasmid size: 3681 bp, see Jacobebbinghaus et al. 2024 for detailed description and application guidelines
Source of Brick: Jacobebbinghaus et al., 2024 https://doi.org/10.1111/tpj.16677
Acceptor plasmid: pAGM1301
Designer / Builder: N. Jacobebbinghaus and T. Baier
pMBS197
$30.00
$30.00
From Elena Kreis, Michael Schroda lab, University of Kaiserslautern, January 2023
Host strain: TOP10
Selection in E. coli: Spectinomycin (50 μg / ml)
Position L0 (Patron et al): B2
Brick name: cp70B-BioID (N-Term, CDS)
Brick type: Reporter gene (CDS)
Brick function: BioID chloroplast targeted by CTP of HSP70B for N-terminal fusion
Source of Brick: Sythesized
Acceptor plasmid: pAGM1276
Designer / Builder: E. Kreis
- «Previous Page
- 1
- …
- 6
- 7
- 8
- 9
- Next Page»