MoClo Level 2 Devices
pK4
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Kanamycin (50 µg/ml)
BsaI sites presence for homology arms cloning: Yes
BbsI sites for Level 2 cloning: Yes: TGCC and GGGA.
LacZα gene flanked by BbsI sites: Yes
Chloroplast 5′ and 3′ homology arms: None. Customized by user by cloning at BsaI sites.
Function: Golden Gate compatible destination vector in a two step reaction: (1) user-chosen Chlamydomonas plastome 5’ and 3’ homology regions (arms) are introduced using BsaI enzyme. (2) Obtained vector is used as a Level 2 Golden Gate compatible destination vector to introduce modules using BbsI enzyme. For more information, refer to Melero-Cobo et al.
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pLM20.K.4-3’L
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Kanamycin (50 µg/ml)
BsaI sites presence for homology arms cloning: No
BbsI sites for Level 2 cloning: Yes: TGCC and GGGA.
LacZα gene flanked by BbsI sites: Yes
Chloroplast 5′ and 3′ homology arms: Amplified from pLM20 vector (Michelet., 2011)
Function: Level 2 Golden Gate compatible destination vector, containing Chlamydomonas plastome 5’ and 3’ homology regions (arms) to allow Chlamydomonas plastome transformation by homologous recombination in the pLM20 locus. For more information, refer to Melero-Cobo et al.
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pMBS431
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: PSADpro::roGFP2-Tsa2ΔCR::3xHA::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-3xHA tag
TU1: pCM1-01
TU2: pMBS425
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS472
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: ARpro::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag
TU1: pCM1-01
TU2: pMBS469
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS473
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: ARpro::roGFP2::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: roGFP2 unfused-no tag
TU1: pCM1-01
TU2: pMBS470
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS650
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, 7/12/21
Name: PSADpro::aadA::PSAD-T:::: ARpro::USPA::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
TU1: pCM1-01
TU2: pMBS642
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS651
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: ARpro::HSP70B::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
TU1: pCM1-01
TU2: pMBS643
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS652
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: ARpro::CDJ1::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
TU1: pCM1-01
TU2: pMBS644
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS653
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: ARpro::PSAN::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the chloroplast (Lumen)
TU1: pCM1-01
TU2: pMBS645
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS654
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: ARpro::PSBO::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the chloroplast (Lumen)
TU1: pCM1-01
TU2: pMBS646
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS655
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: ARpro::HSP70C::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the mitochondria
TU1: pCM1-01
TU2: pMBS647
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS656
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: ARpro::BIP1::roGFP2-Tsa2ΔCR::ER-ret::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the endoplasmic reticulum
TU1: pCM1-01
TU2: pMBS648
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS657
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: ARpro::roGFP2-Tsa2ΔCR::SV40::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the nucleus
TU1: pCM1-01
TU2: pMBS649
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS720
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::aadA::PSAD-T:::: ARpro::roGFP2-Orp1::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag
TU1: pCM1-01
TU2: pMBS719
End Linker: pICH41744-pL2-EL2
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pWF.K.4
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Kanamycin (50 µg/ml)
BsaI sites presence for homology arms cloning: No
BbsI sites for Level 2 cloning: Yes: TGCC and GGGA.
LacZα gene flanked by BbsI sites: Yes
Chloroplast 5′ and 3′ homology arms: Amplified from pWF vector (Kuras and Wollman, 1994)
Function: Level 2 Golden Gate compatible destination vector, containing Chlamydomonas plastome 5’ and 3’ homology regions (arms) to allow Chlamydomonas plastome transformation by homologous recombination in the pWF locus. For more information, refer to Melero-Cobo et al.
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.