MoClo-related GOIs +

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, March 2022

Host strain: TOP10
Selection in E. coli: kanamycin (50 μg/ ml)
Position L0 (Patron et al):
Brick name: L2-Ble-CRed (BleoR)
Brick type: Destination vector
Sequence reference:
Brick function: Destination vector with L1 ble (BleoR) and CRed accepting L0 parts
Source of Brick: Niemeyer and Schroda 2022
Acceptor plasmid: pAGM4673
Designer / Builder: J. Niemeyer

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

Sequence

Niemeyer J, Schroda M. New destination vectors facilitate Modular Cloning for Chlamydomonas. Curr Genet. 2022 Aug;68(3-4):531-536. doi: 10.1007/s00294-022-01239-x. Epub 2022 Apr 16. PMID: 35429260; PMCID: PMC9279246.