MoClo Toolkit Archives - Page 10 of 11 - Chlamydomonas Resource Center

* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $1000.00. Subscriptions cannot be used for CLiP mutants, BACs nor fosmids.

pMBS515

$30.00

From Elena Kreis, Michael Schroda lab, University of Kaiserslautern, January 2023

Host strain: TOP10
Selection in E. coli: Spectinomycin (50 μg / ml)
Position L0 (Patron et al): B2
Brick name: cp70B-TurboID/cp70B-TID (N-Term, CDS)
Brick type: Reporter gene (CDS)
Brick function: TurboID/TID chloroplast targeted by CTP of HSP70B for N-terminal fusion
Source of Brick: Sythesized
Acceptor plasmid: pAGM1276
Designer / Builder: E. Kreis

pMBS527

$30.00

From Elena Kreis, Michael Schroda lab, University of Kaiserslautern, January 2023

Host strain: TOP10
Selection in E. coli: Spectinomycin (50 μg / ml)
Position L0 (Patron et al): B5
Brick name: APEX2 (C-Term, CDS)
Brick type: Reporter gene (CDS)
Brick function: APEX2 without STOP for C-terminal fusion
Source of Brick: Sythesized
Acceptor plasmid: pAGM1276
Designer / Builder: E. Kreis

pMBS639

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B2
Brick name: CTP HSP70B (N-ter, CDS)
Brick type: Signal and Targeting peptides (CDS)
Brick function: Allows the targetting to the Chloroplast
Source of Brick: Cre06.g250100.t1.2
Acceptor plasmid: pAGM1276
Designer / Builder: J. Niemeyer

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M. Real-time Monitoring of Subcellular H2O2 Distribution in Chlamydomonas reinhardtii. Plant Cell. 2021 Jul 1:koab176. doi: 10.1093/plcell/koab176. Epub ahead of print. PMID: 34196712.

pMBS640

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B2
Brick name: CTP CDJ1 (N-ter, CDS)
Brick type: Signal and Targeting peptides (CDS)
Brick function: Allows the targetting to the Chloroplast
Source of Brick: Cre12.g507650
Acceptor plasmid: pAGM1276
Designer / Builder: J. Niemeyer

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M. Real-time Monitoring of Subcellular H2O2 Distribution in Chlamydomonas reinhardtii. Plant Cell. 2021 Jul 1:koab176. doi: 10.1093/plcell/koab176. Epub ahead of print. PMID: 34196712.

pMBS641

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B2
Brick name: CTP PSBO (N-ter, CDS)
Brick type: Signal and Targeting peptides (CDS)
Brick function: Allows the targetting to the Chloroplast
Source of Brick: Cre09.g396213
Acceptor plasmid: pAGM1276
Designer / Builder: J. Niemeyer

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M. Real-time Monitoring of Subcellular H2O2 Distribution in Chlamydomonas reinhardtii. Plant Cell. 2021 Jul 1:koab176. doi: 10.1093/plcell/koab176. Epub ahead of print. PMID: 34196712.

pMBS642

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Name: ARpro::USPA::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pCM0-053
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pMBS643

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Name: ARpro::HSP70B::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pMBS639
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pMBS644

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Name: ARpro::CDJ1::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pMBS640
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pMBS645

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Name: ARpro::PSAN::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the chloroplast (Lumen)
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pMBS298
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pMBS646

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Name: ARpro::PSBO::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the chloroplast (Lumen)
Brick 1 – pL0: pCM0-011 Brick 2 – pL0: pMBS641
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pMBS647

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Name: ARpro::HSP70C::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the mitochondria
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pCM0-057
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pMBS648

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Name: ARpro::BIP1::roGFP2-Tsa2ΔCR::ER-ret::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the endoplasmic reticulum
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pCM0-056
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-111
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pMBS649

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Name: ARpro::roGFP2-Tsa2ΔCR::SV40::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the nucleus
Brick 1 – pL0: pCM0-017
Brick 2 – pL0: pMBS419
Brick 3 – pL0: pCM0-109
Brick 4 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pMBS650

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, 7/12/21

Name: PSADpro::aadA::PSAD-T:::: ARpro::USPA::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
TU1: pCM1-01
TU2: pMBS642
End Linker: pICH41744-pL2-EL2

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pMBS651

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Name: PSADpro::aadA::PSAD-T:::: ARpro::HSP70B::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
TU1: pCM1-01
TU2: pMBS643
End Linker: pICH41744-pL2-EL2

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

pMBS652

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021

Name: PSADpro::aadA::PSAD-T:::: ARpro::CDJ1::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg/ ml)
Original pL2: pAGM4673
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
TU1: pCM1-01
TU2: pMBS644
End Linker: pICH41744-pL2-EL2

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

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