MoClo Level 0 Parts
pCC0-1
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Chloramphenicol (17µg/ml)
Position L0 (Patron et al): A1-B1
Brick name: PrbcL
Brick type: Promoter + 5’UTR
Brick function: Add rbcL promoter, 5’UTR and first 27 rbcL gene aminoacids
Source of Brick: Melero-Cobo et al., 2025
Acceptor plasmid: Twist vector_High copy, ChloroR
Designer / Builder: Melero-Cobo & Gallemí
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC0-10
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Chloramphenicol (17µg/ml)
Position L0 (Patron et al): B6-C1
Brick name: TpsbA·T7
Brick type: 3’UTR + Terminator
Brick function: Add psbA terminator fused to T7 terminator
Source of Brick: Melero-Cobo et al., 2025
Acceptor plasmid: Twist vector_High copy, ChloroR
Designer / Builder: Melero-Cobo & Gallemí
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC0-11
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Chloramphenicol (17µg/ml)
Position L0 (Patron et al): B6-C1
Brick name: TatpB
Brick type: 3’UTR + Terminator
Brick function: Add atpB terminator
Source of Brick: Melero-Cobo et al., 2025
Acceptor plasmid: Twist vector_High copy, ChloroR
Designer / Builder: Melero-Cobo & Gallemí
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC0-12
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Chloramphenicol (17µg/ml)
Position L0 (Patron et al): B6-C1
Brick name: TpsaA
Brick type: 3’UTR + Terminator
Brick function: Add psaA terminator
Source of Brick: Melero-Cobo et al., 2025
Acceptor plasmid: Twist vector_High copy, ChloroR
Designer / Builder: Melero-Cobo & Gallemí
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC0-16
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Chloramphenicol (17µg/ml)
Position L0 (Patron et al): A1-B1
Brick name: PpsbD
Brick type: Promoter + 5’UTR
Brick function: Add psbD promoter and 5’UTR
Source of Brick: Melero-Cobo et al., 2025
Acceptor plasmid: Twist vector_High copy, ChloroR
Designer / Builder: Melero-Cobo & Gallemí
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC0-3
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Chloramphenicol (17µg/ml)
Position L0 (Patron et al): A1-B1
Brick name: P16SrRNA
Brick type: Promoter + 5’UTR
Brick function: Add 16SrRNA promoter and rbcL 5’UTR
Source of Brick: Melero-Cobo et al., 2025
Acceptor plasmid: Twist vector_High copy, ChloroR
Designer / Builder: Melero-Cobo & Gallemí
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC0-35
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Chloramphenicol (17µg/ml)
Position L0 (Patron et al): B6-C1
Brick name: TsrbcL
Brick type: 3’UTR + Terminator
Brick function: Add a short version of the rbcL terminator
Source of Brick: Melero-Cobo et al., 2025
Acceptor plasmid: Twist vector_High copy, ChloroR
Designer / Builder: Melero-Cobo & Gallemí
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC0-5
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Chloramphenicol (17µg/ml)
Position L0 (Patron et al): A1-B1
Brick name: PatpB
Brick type: Promoter + 5’UTR
Brick function: Add atpB promoter and 5’UTR
Source of Brick: Melero-Cobo et al., 2025
Acceptor plasmid: Twist vector_High copy, ChloroR
Designer / Builder: Melero-Cobo & Gallemí
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC0-6
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Chloramphenicol (17µg/ml)
Position L0 (Patron et al): A1-B1
Brick name: PpsaA
Brick type: Promoter + 5’UTR
Brick function: Add psaA promoter and 5’UTR
Source of Brick: Melero-Cobo et al., 2025
Acceptor plasmid: Twist vector_High copy, ChloroR
Designer / Builder: Melero-Cobo & Gallemí
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC0-9
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Chloramphenicol (17µg/ml)
Position L0 (Patron et al): B2-B5
Brick name: CDS uidA
Brick type: Reporter gene (CDS)
Brick function: Add uidA reporter gene sequence from E. coli
Source of Brick: Melero-Cobo et al., 2025
Acceptor plasmid: Twist vector_High copy, ChloroR
Designer / Builder: Melero-Cobo & Gallemí
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCM0_TB011
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: DH5a
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B4
Brick name: mVenus (2xi1) (CDS)
Brick type: Reporter gene (CDS)
Brick function: mVenus reporter (with 2 copies of the RBCS2 intron 1) + GSG-Linkers in B4
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pAGM1299
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0_TB030
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: DH5a
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B4
Brick name: Clover (2xi1) (CDS)
Brick type: Reporter gene (CDS)
Brick function: Clover reporter (with 2 copies of the RBCS2 intron 1) + GSG-Linkers in B4
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pAGM1299
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0_TB032
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: DH5a
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B4
Brick name: mRuby2 (2xi1) (CDS)
Brick type: Reporter gene (CDS)
Brick function: mRuby2 reporter (with 2 copies of the RBCS2 intron 1) + GSG-Linkers in B4
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pAGM1299
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0_TB041
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: DH5a
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3-B5
Brick name: aada (2xi1) (SpecR, CDS)
Brick type: Antibiotic Resistance gene (CDS)
Brick function: aadA confering resistance to Spectinomycine in Chlamy, codon optimized for Chlamy, including 2 copies of the rbcS2i1
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41308
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0_TB043
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: DH5a
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B4
Brick name: aada (2xi1) (SpecR, CDS)
Brick type: Antibiotic Resistance gene (CDS)
Brick function: aadA confering resistance to Spectinomycine in Chlamy, codon optimized for Chlamy, including 2 copies of the rbcS2i1
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pAGM1299
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
pCM0_TB057
$30.00
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: DH5a
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): A1-B1
Brick name: PSAD_mut-SspI (Pro + 5’UTR)
Brick type: promoter + 5’UTR
Brick function: PSAD promoter with mutated SspI site for L2 usage
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pAGM1251
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.
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