MoClo Level 0 Parts
pMBS298
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B2
Brick name: CTP PSAN (N-ter, CDS)
Brick type: Signal and Targeting peptides (CDS)
Brick function: Allows the targetting to the Chloroplast
Source of Brick: Cre02.g082500
Acceptor plasmid: pAGM1276
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M. Real-time Monitoring of Subcellular H2O2 Distribution in Chlamydomonas reinhardtii. Plant Cell. 2021 Jul 1:koab176. doi: 10.1093/plcell/koab176. Epub ahead of print. PMID: 34196712.
pMBS418
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B3-B4
Brick name: roGFP2-Orp1 (CDS)
Brick type: Reporter gene (CDS)
Brick function: roGFP2-Orp1 in CDS without STOP and that can be fused in C-Ter
Source of Brick: Synthetized
Acceptor plasmid: pAGM1287
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M. Real-time Monitoring of Subcellular H2O2 Distribution in Chlamydomonas reinhardtii. Plant Cell. 2021 Jul 1:koab176. doi: 10.1093/plcell/koab176. Epub ahead of print. PMID: 34196712.
pMBS419
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B3-B4
Brick name: roGFP2-Tsa2ΔCR (CDS)
Brick type: Reporter gene (CDS)
Brick function: roGFP2-Tsa2ΔCR in CDS without STOP and that can be fused in C-Ter
Source of Brick: Synthetized
Acceptor plasmid: pAGM1287
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M. Real-time Monitoring of Subcellular H2O2 Distribution in Chlamydomonas reinhardtii. Plant Cell. 2021 Jul 1:koab176. doi: 10.1093/plcell/koab176. Epub ahead of print. PMID: 34196712.
pMBS454
$30.00
$30.00
From Elena Kreis, Michael Schroda lab, University of Kaiserslautern, January 2023
Host strain: TOP10
Selection in E. coli: Spectinomycin (50 μg / ml)
Position L0 (Patron et al): B2
Brick name: cp70B-APEX2 (N-Term, CDS)
Brick type: Reporter gene (CDS)
Brick function: APEX2 chloroplast targeted by CTP of HSP70B for N-terminal fusion
Source of Brick: Sythesized
Acceptor plasmid: pAGM1276
Designer / Builder: E. Kreis
pMBS467
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B3-B4
Brick name: roGFP2
Brick type: Reporter gene (CDS)
Brick function: roGFP2 in CDS without STOP and that can be fused in C-Ter
Source of Brick: PCR on pMBS419
Acceptor plasmid: pAGM1287
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M. Real-time Monitoring of Subcellular H2O2 Distribution in Chlamydomonas reinhardtii. Plant Cell. 2021 Jul 1:koab176. doi: 10.1093/plcell/koab176. Epub ahead of print. PMID: 34196712.
pMBS512
$30.00
$30.00
From Elena Kreis, Michael Schroda lab, University of Kaiserslautern, January 2023
Host strain: TOP10
Selection in E. coli: Kanamycin (50 μg / ml)
Position L0 (Patron et al): B5
Brick name: TurboID/TID (C-Term, CDS)
Brick type: Reporter gene (CDS)
Brick function: TurboID/TID without STOP for C-terminal fusion
Source of Brick: Sythesized
Acceptor plasmid: pUC57-Kan-BsaI/BpiI-free
Designer / Builder: E. Kreis
pMBS515
$30.00
$30.00
From Elena Kreis, Michael Schroda lab, University of Kaiserslautern, January 2023
Host strain: TOP10
Selection in E. coli: Spectinomycin (50 μg / ml)
Position L0 (Patron et al): B2
Brick name: cp70B-TurboID/cp70B-TID (N-Term, CDS)
Brick type: Reporter gene (CDS)
Brick function: TurboID/TID chloroplast targeted by CTP of HSP70B for N-terminal fusion
Source of Brick: Sythesized
Acceptor plasmid: pAGM1276
Designer / Builder: E. Kreis
pMBS527
$30.00
$30.00
From Elena Kreis, Michael Schroda lab, University of Kaiserslautern, January 2023
Host strain: TOP10
Selection in E. coli: Spectinomycin (50 μg / ml)
Position L0 (Patron et al): B5
Brick name: APEX2 (C-Term, CDS)
Brick type: Reporter gene (CDS)
Brick function: APEX2 without STOP for C-terminal fusion
Source of Brick: Sythesized
Acceptor plasmid: pAGM1276
Designer / Builder: E. Kreis
pMBS639
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B2
Brick name: CTP HSP70B (N-ter, CDS)
Brick type: Signal and Targeting peptides (CDS)
Brick function: Allows the targetting to the Chloroplast
Source of Brick: Cre06.g250100.t1.2
Acceptor plasmid: pAGM1276
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M. Real-time Monitoring of Subcellular H2O2 Distribution in Chlamydomonas reinhardtii. Plant Cell. 2021 Jul 1:koab176. doi: 10.1093/plcell/koab176. Epub ahead of print. PMID: 34196712.
pMBS640
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B2
Brick name: CTP CDJ1 (N-ter, CDS)
Brick type: Signal and Targeting peptides (CDS)
Brick function: Allows the targetting to the Chloroplast
Source of Brick: Cre12.g507650
Acceptor plasmid: pAGM1276
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M. Real-time Monitoring of Subcellular H2O2 Distribution in Chlamydomonas reinhardtii. Plant Cell. 2021 Jul 1:koab176. doi: 10.1093/plcell/koab176. Epub ahead of print. PMID: 34196712.
pMBS641
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B2
Brick name: CTP PSBO (N-ter, CDS)
Brick type: Signal and Targeting peptides (CDS)
Brick function: Allows the targetting to the Chloroplast
Source of Brick: Cre09.g396213
Acceptor plasmid: pAGM1276
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Niemeyer J, Scheuring D, Oestreicher J, Morgan B, Schroda M. Real-time Monitoring of Subcellular H2O2 Distribution in Chlamydomonas reinhardtii. Plant Cell. 2021 Jul 1:koab176. doi: 10.1093/plcell/koab176. Epub ahead of print. PMID: 34196712.
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