MoClo Level 1 Modules
pCC1-05
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
TU short name: PatpA·aadA·TrbcL
TU full name: pL1-2F-atpApro::aadA::TrbcL
Host strain: DH5α
Selection in E. coli: Ampicillin (100µg/ml)
Type: 2F
Original pl1: pICH47742
Function: Resistance in Chlamy chloroplast to Spectinomycin
Source of the module: Melero-Cobo et al., 2025
Designer/ Builder: Melero-Cobo & Gallemí
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
Comment: This module was not obtained by Golden Gate. Refer to Melero-Cobo et al. for further details.
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC1-23
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
TU short name: PatpA·aadA·TrbcL
TU full name: pL1-1F-atpApro::aadA::TrbcL
Host strain: DH5α
Selection in E. coli: Ampicillin (100µg/ml)
Type: 1F
Original pl1: pICH47732
Function: Resistance in Chlamy chloroplast to Spectinomycin
Source of the module: Melero-Cobo et al., 2025
Designer/ Builder: Melero-Cobo & Gallemí
Fusion 5′ lvl2: TGCC
Fusion 3′ lvl2: GCAA
Comment: This module was not obtained by Golden Gate. Refer to Melero-Cobo et al. for further details.
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC1-24
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
TU short name: PatpA·aadA·TrbcL
TU full name: pL1-3F-atpApro::aadA::TrbcL
Host strain: DH5α
Selection in E. coli: Ampicillin (100µg/ml)
Type: 3F
Original pl1: pICH47751
Function: Resistance in Chlamy chloroplast to Spectinomycin
Source of the module: Melero-Cobo et al., 2025
Designer/ Builder: Melero-Cobo & Gallemí
Fusion 5′ lvl2: ACTA
Fusion 3′ lvl2: TTAC
Comment: This module was not obtained by Golden Gate. Refer to Melero-Cobo et al. for further details.
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCC1-25
$30.00
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
TU short name: PatpA·aadA·TrbcL
TU full name: pL1-4F-atpApro::aadA::TrbcL
Host strain: DH5α
Selection in E. coli: Ampicillin (100µg/ml)
Type: 4F
Original pl1: pICH47761
Function: Resistance in Chlamy chloroplast to Spectinomycin
Source of the module: Melero-Cobo et al., 2025
Designer/ Builder: Melero-Cobo & Gallemí
Fusion 5′ lvl2: TTAC
Fusion 3′ lvl2: CAGA
Comment: This module was not obtained by Golden Gate. Refer to Melero-Cobo et al. for further details.
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.
pCM1-029
$30.00
$30.00
From Félix de Carpentier, Institute of Physico-Chemical Biology-Paris, February 2020
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018) and de Carpentier et al. (2020).
Host strain: NEB® 10-beta
Selection in E. coli: ampicillin (50 μg/ ml)
Position L1 (Weber et al): 2F
Brick name: PAR-BSR-TRBCS2 (TU)
Brick type: Antibiotic Resistance TU
Brick function: Resistance to Blasticidin in Chlamy
Source of Brick: Assembly of pCM0-020, pCM0-120 and pCM0-115
Acceptor plasmid: pICH47742
Designer / Builder: F. de Carpentier and J. Le Peillet
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
de Carpentier et al. (2020) Blasticidin S deaminase: a new efficient selectable marker for Chlamydomonas reinhardtii. Frontiers in Plant Science 11:242
de Carpentier F, Le Peillet J, Boisset ND, Crozet P, Lemaire SD, Danon A. Blasticidin S Deaminase: A New Efficient Selectable Marker for Chlamydomonas reinhardtii. Front Plant Sci. 2020 Mar 5;11:242. doi: 10.3389/fpls.2020.00242. PMID: 32211000; PMCID: PMC7066984.
pMBS425
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: PSADpro::roGFP2-Tsa2ΔCR::3xHA::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-3xHA tag
Brick 1 – pL0: pCM0-016
Brick 2 – pL0: pMBS419
Brick 3 – pL0: pCM0-093
Brick 4 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS469
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: ARpro::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag
Brick 1 – pL0: pCM0-017
Brick 2 – pL0: pMBS419
Brick 3 – pL0: pCM0-101
Brick 4 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS470
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: ARpro::roGFP2::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: roGFP2 unfused-no tag
Brick 1 – pL0: pCM0-017
Brick 2 – pL0: pMBS467
Brick 3 – pL0: pCM0-101
Brick 4 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS642
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: ARpro::USPA::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pCM0-053
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS643
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: ARpro::HSP70B::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pMBS639
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS644
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: ARpro::CDJ1::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the chloroplast (Stroma)
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pMBS640
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS645
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: ARpro::PSAN::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the chloroplast (Lumen)
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pMBS298
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS646
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: ARpro::PSBO::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the chloroplast (Lumen)
Brick 1 – pL0: pCM0-011 Brick 2 – pL0: pMBS641
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS647
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: ARpro::HSP70C::roGFP2-Tsa2ΔCR::MStop::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the mitochondria
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pCM0-057
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-101
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS648
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: ARpro::BIP1::roGFP2-Tsa2ΔCR::ER-ret::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the endoplasmic reticulum
Brick 1 – pL0: pCM0-011
Brick 2 – pL0: pCM0-056
Brick 3 – pL0: pMBS419
Brick 4 – pL0: pCM0-111
Brick 5 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
pMBS649
$30.00
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Name: ARpro::roGFP2-Tsa2ΔCR::SV40::RPL23-T
Host strain: TOP10
Selection in E. coli: ampicillin (100 μg/ ml)
Type: 2F
Original pl1: pICH47742
Function: H2O2 reporter-no tag targeted to the nucleus
Brick 1 – pL0: pCM0-017
Brick 2 – pL0: pMBS419
Brick 3 – pL0: pCM0-109
Brick 4 – pL0: pCM0-119
Fusion 5′ lvl2: GCAA
Fusion 3′ lvl2: ACTA
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/