Venus & FLAG-tagged Plasmids for Chlamydomonas Spatial Interactome (CSI) Collection, Wang/Jonikas 2022

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820

From Martin Jonikas, Princeton University, March 2023

This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.

Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin

A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820