Chlamydomonas Spatial Interactome (CSI) Collection, Wang/Jonikas 2022

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre14.g614800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre16.g674800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre16.g691500-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g509650-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PDS1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre04.g212350-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre16.g661150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: CGL5

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g285500-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g197750-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: GPX3

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre10.g464600-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: CAM14

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre14.g630000-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: KIR2

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g496700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: TRX17

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g195850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre01.g043450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g091250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g256850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre07.g320400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g269752-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: TRX15

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre17.g721150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g091300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre09.g405050-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: ATPvD2

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre16.g651250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre14.g624500-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: RRP46

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre01.g048750-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PPR7

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g279850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g169900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g181250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: SNE18/CGLD13

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g252150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PGP6

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g207000-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre09.g387134-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre09.g409700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –