Chlamydomonas Spatial Interactome (CSI) Collection, Wang/Jonikas 2022

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g491050-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: NSG2/RIR2A/RIR2

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre05.g233900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: APX4

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre16.g663900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PBGD1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre13.g604700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g509350-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: FAP238

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre01.g044400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre04.g221450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g531850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g085450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: CPX1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g490050-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: GMT1/TPT18

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g191300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: LCI34/LCI35

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g090400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre10.g417700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: RPL3

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre10.g439050-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: MITC7

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g143635-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g145050-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: CMK1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g107300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: DPS1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g522950-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: MIND1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre01.g012300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g298000-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre16.g657350-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g108300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre17.g731571-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre17.g729950-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: SRX1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g544150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: CYN7

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g171179-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g155250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g167850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre04.g217946-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g073700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: UPD3/UROD3