Chlamydomonas Spatial Interactome (CSI) Collection, Wang/Jonikas 2022

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g284600-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: RBD2

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g108850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PRPL17

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g195650-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PRPS10

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre05.g242400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PGR5

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre09.g397400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: SDR17

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre08.g365900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: LHCSR1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g189300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PLP10/PLAP10

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre09.g386732-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g252550-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: DLA3

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre13.g565550-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: RPB11

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre01.g028150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: CPLD69

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre16.g669650-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g257450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: ATPVL1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre01.g027000-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: RPL11

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre14.g616900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: SLP2/SULP2

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g088900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PRPL1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre10.g436050-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: FSD1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g256250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: TEF14

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre11.g467755-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre16.g677450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre13.g570851-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre10.g449550-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PRX3

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre10.g438100-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PGP2

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre01.g030250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g250250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: ATPVC

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g143887-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g305000-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g483650-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g204250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: SAH1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g115150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –