Chlamydomonas Spatial Interactome (CSI) Collection, Wang/Jonikas 2022

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre13.g602850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: SRP23

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g295400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: MITC11

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre10.g422300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PRX6

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre07.g356400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: POC7/RPD1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g199900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: EIF4E

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g103550-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: EIF1A

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g272200-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre07.g320450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g256800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g530500-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre08.g376300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre01.g009150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre01.g020350-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: SDH3

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre07.g327400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: NUO9

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre14.g611150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre12.g557900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: CDI1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g088000-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PHB1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre14.g615050-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g155650-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: CLC1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g141250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PPP10

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g292550-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: PP1A/PP1C/PPP25

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g272900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: AOF3/EZY10

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre07.g315100-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre16.g683250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre07.g321750-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g308950-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: –

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre03.g157700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: COX5C

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre02.g092900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: GUA1

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre06.g309350-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: ERD2C

From Martin Jonikas, Princeton University, May 2022

cMJ030 expressing pLM005-Cre01.g002300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).

Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.

To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.

Protein Name: CYN4/CYN19-2