Chlamydomonas Spatial Interactome (CSI) Collection, Mackinder/Jonikas 2017

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre16.g661750-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre17.g698400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre01.g014350-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre17.g734500-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre10.g435100-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre06.g263550-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g507300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g162850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre07.g321800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre07.g330250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g486250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g552450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre09.g402219-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g179800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre14.g626700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre13.g592400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre06.g275000-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre06.g271800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g191250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre10.g444700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre02.g097800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre02.g144800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre06.g260450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre17.g721500-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre06.g283750-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g164600-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g181200-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre02.g120150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g187450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g487900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.