Chlamydomonas Spatial Interactome (CSI) Collection, Mackinder/Jonikas 2017

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre07.g337100-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre08.g362900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g509050-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre17.g740950-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre10.g427250-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g181600-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre10.g439350-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g181500-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g189800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre01.g030900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre16.g683600-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre07.g313800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre07.g335700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre07.g344260-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g528750-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g554850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g156600-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre16.g662150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre06.g258850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre17.g744547-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g210961-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre02.g078507-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre16.g661700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g146287-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g524950-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre11.g467759-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre08.g360200-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre06.g306300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre13.g584850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre07.g336350-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.