Chlamydomonas Spatial Interactome (CSI) Collection, Mackinder/Jonikas 2017
CSI_FC2D10
$30.00
$30.00
From Martin Jonikas, Princeton University, April 2017
cMJ030 expressing pLM005-Cre12.g519500-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).
Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .
To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.
CSI_FC2D11
$30.00
$30.00
From Martin Jonikas, Princeton University, April 2017
cMJ030 expressing pLM005-Cre07.g339900-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).
Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .
To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.
CSI_FC2D12
$30.00
$30.00
From Martin Jonikas, Princeton University, April 2017
cMJ030 expressing pLM005-Cre16.g671350-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).
Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .
To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.
CSI_FC2E01
$30.00
$30.00
From Martin Jonikas, Princeton University, April 2017
cMJ030 expressing pLM005-Cre09.g389615-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).
Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .
To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.
CSI_FC2E02
$30.00
$30.00
From Martin Jonikas, Princeton University, April 2017
cMJ030 expressing pLM005-Cre06.g295450-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).
Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://chlamylibrary.org .
To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.
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