CLiP Strains

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) g5042, chromosome_4 base 3885738 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • g5042
  • Chromosome:
  • 4

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) intergenic, chromosome_2 base 509468 (genome v5.3) – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • intergenic
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre09.g410500, chromosome_9 base 6948046 (genome v5.3), multiple splice variants – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre09.g410500
  • Chromosome:
  • 9

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) g4550, chromosome_4 base 409683 (genome v5.3), intron – WARNING position has 75% probability of being correct
2) g4550, chromosome_4 base 409684 (genome v5.3), intron – WARNING position has 75% probability of being correct
3) Cre10.g438800, chromosome_10 base 2765209 (genome v5.3), multiple splice variants – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre10.g438800, g4550
  • Chromosome:
  • 10, 4

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre02.g113850, chromosome_2 base 6201387 (genome v5.3), intron – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre02.g113850
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) intergenic, chromosome_11 base 1135895 (genome v5.3) – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • intergenic
  • Chromosome:
  • 11

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre16.g649100, chromosome_16 base 1015680 (genome v5.3), CDS – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre16.g649100
  • Chromosome:
  • 16

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre02.g113450, chromosome_2 base 6138223 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre02.g113450
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre03.g150000, chromosome_3 base 1258493 (genome v5.3), CDS – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre03.g150000
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre01.g022681, chromosome_1 base 3535409 (genome v5.3), multiple splice variants – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre01.g022681
  • Chromosome:
  • 1

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre12.g500550, chromosome_12 base 3051042 (genome v5.3), intron – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre12.g500550
  • Chromosome:
  • 12

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) g4466, chromosome_3 base 9083034 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • g4466
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre05.g241150, chromosome_5 base 3399423 (genome v5.3), intron – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre05.g241150
  • Chromosome:
  • 5

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre13.g584200, chromosome_13 base 2996408 (genome v5.3), intron – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre13.g584200
  • Chromosome:
  • 13

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre12.g560900, chromosome_12 base 6884557 (genome v5.3), CDS – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre12.g560900
  • Chromosome:
  • 12

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) g16601, chromosome_16 base 6108658 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • g16601
  • Chromosome:
  • 16

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre06.g297900, chromosome_6 base 7211978 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre06.g297900
  • Chromosome:
  • 6

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) DHC11 (Cre12.g555950), chromosome_12 base 7512794 (genome v5.3), intron – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • DHC11
  • Chromosome:
  • 12

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre08.g369000, chromosome_8 base 2131133 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre08.g369000
  • Chromosome:
  • 8

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre07.g339104, chromosome_7 base 3904728 (genome v5.3), multiple splice variants – WARNING position has 75% probability of being correct
2) Cre07.g339104, chromosome_7 base 3904764 (genome v5.3), multiple splice variants – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre07.g339104
  • Chromosome:
  • 7

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) PTK12 (Cre07.g351800), chromosome_7 base 5586941 (genome v5.3), multiple splice variants – WARNING position has 75% probability of being correct
2) CGL56 (Cre05.g247450), chromosome_5 base 897042 (genome v5.3), intron – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • CGL56, PTK12
  • Chromosome:
  • 5, 7

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre02.g110250, chromosome_2 base 5732258 (genome v5.3), intron – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre02.g110250
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre12.g559850, chromosome_12 base 7005975 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre12.g559850
  • Chromosome:
  • 12

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre02.g112250, chromosome_2 base 5998852 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre02.g112250
  • Chromosome:
  • 2

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre07.g355950, chromosome_7 base 6150353 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre07.g355950
  • Chromosome:
  • 7

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) g6239, chromosome_6 base 3463235 (genome v5.3), multiple splice variants – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • g6239
  • Chromosome:
  • 6

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre03.g159350, chromosome_3 base 2444833 (genome v5.3), 5’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre03.g159350
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre13.g575450, chromosome_13 base 1860168 (genome v5.3), multiple splice variants – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre13.g575450
  • Chromosome:
  • 13

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre03.g169450, chromosome_3 base 3706603 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre03.g169450
  • Chromosome:
  • 3

If you use this mutant for your work, please cite: Li, Zhang, Patena et al. 2016 Plant Cell.

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre14.g621000, chromosome_14 base 1927972 (genome v5.3), 3’UTR – WARNING position has 75% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

  • Locus:
  • Cre14.g621000
  • Chromosome:
  • 14