Strains
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner from the Hegemann lab suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner from the Hegemann lab suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner from the Hegemann lab suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner from the Hegemann lab suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner from the Hegemann lab suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Sizova I, Greiner A, Awasthi M, Kateriya S, Hegemann P (2013) Nuclear gene targeting in Chlamydomonas using engineered zinc-finger nucleases. Plant J 73:873-82
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Sizova I, Greiner A, Awasthi M, Kateriya S, Hegemann P (2013) Nuclear gene targeting in Chlamydomonas using engineered zinc-finger nucleases. Plant J 73:873-82
From Irina Sizova, Hegemann lab, Humboldt University-Berlin, November 2015
Sizova I, Greiner A, Awasthi M, Kateriya S, Hegemann P (2013) Nuclear gene targeting in Chlamydomonas using engineered zinc-finger nucleases. Plant J 73:873-82
From Kelly Bower, Porter Lab, University of Minnesota, December 2015
sup-pf-4 mt- (CC-2366) rescued with FAP155 that was carboxyl terminally tagged with SNAP by transformation with pSI103 with selection on paromomycin.
Gui L, Song K, Tritschler D, Bower R, Yan S, Dai A, Augspurger K, Sakizadeh J, Grzemska M, Ni T, Porter ME, Nicastro D. Scaffold subunits support associated subunit assembly in the Chlamydomonas ciliary nexin-dynein regulatory complex. Proc Natl Acad Sci U S A. 2019 Nov 12;116(46):23152-23162. doi: 10.1073/pnas.1910960116. Epub 2019 Oct 28. PMID: 31659045; PMCID: PMC6859327.
From Kelly Bower, Porter Lab, University of Minnesota, December 2015
sup-pf-4 mt- (CC-2366) rescued with FAP155 that was amino terminally tagged with BCCP by transformation with pSI103 with selection on paromomycin.
Gui L, Song K, Tritschler D, Bower R, Yan S, Dai A, Augspurger K, Sakizadeh J, Grzemska M, Ni T, Porter ME, Nicastro D. Scaffold subunits support associated subunit assembly in the Chlamydomonas ciliary nexin-dynein regulatory complex. Proc Natl Acad Sci U S A. 2019 Nov 12;116(46):23152-23162. doi: 10.1073/pnas.1910960116. Epub 2019 Oct 28. PMID: 31659045; PMCID: PMC6859327.
From Kelly Bower, Porter Lab, University of Minnesota, December 2015
ida8-1 mt- (CC-4089) rescued with FAP57 amino terminally tagged with SNAP by transformation with pSI103 with selection on paromomycin.
Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.
From Kelly Bower, Porter Lab, University of Minnesota, December 2015
ida8-1 mt- (CC-4089) rescued with FAP57 carboxyl terminally tagged with SNAP by transformation with pSI103 with selection on paromomycin.
Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.
From Kelly Bower, Porter Lab, University of Minnesota, December 2015
pf18-KAP-GFP transformed with carboxyl terminally tagged mCherry LIC and pNOR1 with selection on Norflurazon.
This strain is paralyzed due to pf18 mutation, and contains both endogenous LIC and KAP, as well as the tagged versions.
From Kelly Bower, Porter Lab, University of Minnesota, December 2015
CC-4487 d1blic [T8D9] rescued with carboxyl terminally tagged mCherry.
Cells are fussy, 2-4 hours in M-N/5 rocking in light seem to produce healthiest flagella, overnight in TAP rocking in light seems to produce an increase in the number of flagellated cells. Sensitive to concentration so use 2X the amount of media as normal for the volume of cells.
The phenotype is mostly paralyzed with some wiggling due to unknown mutation(s), the tagged d1blic gene rescues the stubby flagella phenotype and flagella are nearly full length
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