Strains
CC-5240 drc3 pf14 [M4]
$30.00
$30.00
From Junya Awata, George Witman lab, University of Massachusetts Medical School, May 2016
This double mutant was created by crossing CC-5238 drc3-1 mt+ [D4] to CC-613 pf14 mt-. This strain has the same phenotype as pf14.
Awata J, Song K, Lin J, King SM, Sanderson MJ, Nicastro D, Witman GB (2015) DRC3 connects the N-DRC to dynein g to regulate flagellar waveform. Mol Biol Cell. 26:2788-2800
CC-5241 DRC3-HA mt+ [D5]
$30.00
$30.00
From Junya Awata, George Witman lab, University of Massachusetts Medical School, May 2016
Transformation of CC-5238 drc3-1 mt+ [D4] with DRC3-HA (gene encoding DRC3 tagged with 3xHA peptides on its C-terminus) resulting in expression of DRC3 with a 3xHA tag on its C-terminus.
Awata J, Song K, Lin J, King SM, Sanderson MJ, Nicastro D, Witman GB (2015) DRC3 connects the N-DRC to dynein g to regulate flagellar waveform. Mol Biol Cell. 26:2788-2800
CC-5242 DRC3-R mt+ [H11-4]
$30.00
$30.00
From Junya Awata, George Witman lab, University of Massachusetts Medical School, May 2016
Transformation of CC-5238 drc3-1 mt+ [D4] with DRC. Phenotype of drc3 (D4) is rescued in this strain.
Awata J, Song K, Lin J, King SM, Sanderson MJ, Nicastro D, Witman GB (2015) DRC3 connects the N-DRC to dynein g to regulate flagellar waveform. Mol Biol Cell. 26:2788-2800
CC-5243 DRC3-SNAP mt+ [A9-1]
$30.00
$30.00
From Junya Awata, George Witman lab, University of Massachusetts Medical School, May 2016
This strain was created by transformation of CC-5238 drc3-1 mt+ [D4] with DRC3-SNAP (gene encoding DRC3 tagged with SNAP on its C-terminus). It expresses DRC3 having a SNAP tag on its C-terminus. Phenotype of drc3 (D4) is rescued in this strain.
Awata J, Song K, Lin J, King SM, Sanderson MJ, Nicastro D, Witman GB (2015) DRC3 connects the N-DRC to dynein g to regulate flagellar waveform. Mol Biol Cell. 26:2788-8000
CC-5244 SNAP-DRC3 mt+ [1D12]
$30.00
$30.00
From Junya Awata, George Witman lab, University of Massachusetts Medical School, May 2016
This strain was created by transformation of CC-5238 drc3-1 mt+ [D4] with SNAP-DRC3 (gene encoding DRC3 tagged with SNAP on its N-terminus). It expresses DRC3 having a SNAP tag on its N-terminus.
Song K, Awata J, Tritschler D, Bower R, Witman GB, Porter ME, Nicastro D (2015) In situ localization of N and C termini of subunits of the flagellar nexin-dynein regulatory complex (N-DRC) using SNAP tag and cryo-electron tomography. J Biol Chem. 290:5341-53
CC-5245 tpg1 mt-
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation; slightly impaired swimming motility
This mutant was created by UV mutagenesis of strain CC-125 (137c).
Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R (2010) Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. Curr Biol. 20:441-5
CC-5246 tpg1 mt+
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation; slightly impaired swimming motility
This mutant was created by UV mutagenesis of strain CC-125 (137c).
1/14/22 – This strain was recreated by Tomohiro Kubo (University of Yamanashi) by backcrossing CC-5245 tpg1 mt- to CC-125 wild type mt+ [137c].
Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R (2010) Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. Curr Biol. 20:441-5
CC-5247 oda2 tpg1
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation and lacks outer arm dynein; complete immotile
This mutant was obtained by crossing oda2 and tpg1.
1/14/22 – This strain was recreated by Miho Antoku (King lab) by crossing CC-5245 tpg1 mt- to CC-2230 oda2 mt+
Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R (2010) Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. Curr Biol. 20:441-5
CC-5248 tpg2-1 mt+
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation; slightly impaired swimming motility (phenotype similar to tpg1)
This mutant was created by UV mutagenesis of strain CC-125 (137c).
Kubo T, Yanagisawa HA, Liu Z, Shibuya R, Hirono M, Kamiya R (2014) A conserved flagella-associated protein in Chlamydomonas, FAP234, is essential for axonemal localization of tubulin polyglutamylase TTLL9. Mol Biol Cell. 25:107-17
CC-5249 tpg2-1 mt-
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation; slightly impaired swimming motility (phenotype similar to tpg1)
This mutant was created by UV mutagenesis of strain CC-125 (137c).
Kubo T, Yanagisawa HA, Liu Z, Shibuya R, Hirono M, Kamiya R (2014) A conserved flagella-associated protein in Chlamydomonas, FAP234, is essential for axonemal localization of tubulin polyglutamylase TTLL9. Mol Biol Cell. 25:107-17
CC-5250 ida4 tpg1 mt-
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation and lacks inner arm dynein a, c, and d; impaired swimming motility
This mutant was obtained by crossing ida4 and tpg1.
Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R (2010) Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. Curr Biol. 20:441-5
Kubo T, Yagi T, Kamiya R (2012) Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex. Cytoskeleton 69:1059-68
CC-5251 ida4 tpg1 mt+
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation and lacks inner arm dynein a, c, and d; impaired swimming motility
This mutant was obtained by crossing ida4 and tpg1.
Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R (2010) Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. Curr Biol. 20:441-5
Kubo T, Yagi T, Kamiya R (2012) Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex. Cytoskeleton 69:1059-68
CC-5252 ida9 tpg1 mt+
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: Reduced axonemal tubulin polyglutamylation and lacks inner arm dynein c; impaired swimming motility
This mutant was obtained by crossing ida9 and tpg1.
Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R (2010) Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. Curr Biol. 20:441-5
Kubo T, Yagi T, Kamiya R (2012) Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex. Cytoskeleton 69:1059-68
CC-5254 fla10-1 tpg2-1
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation, shortens its flagella at 32℃ ; slightly impaired swimming motility
This mutant was obtained by crossing fla10-1 and tpg2-1.
Kubo T, Hirono M, Aikawa T, Kamiya R, Witman GB (2015) Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Mol Biol Cell. 26:2810-22
CC-5255 pf28 pf30 tpg1 [1G]
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation, lacks outer arm dynein and inner arm dynein f/I1; generates wild-type length flagella but complete immotile
This mutant was obtained by crossing pf28,pf30 and tpg1.
Kubo T, Hirono M, Aikawa T, Kamiya R, Witman GB (2015) Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Mol Biol Cell. 26:2810-22
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation, lacks outer arm dynein and inner arm dynein f/I1; generates wild-type length flagella but complete immotile
This mutant was obtained by crossing pf28,pf30 and tpg2-1.
Kubo T, Hirono M, Aikawa T, Kamiya R, Witman GB (2015) Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Mol Biol Cell. 26:2810-22
CC-5257 tpg2-3 / ssh1
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation; slightly impaired swimming motility
This mutant was obtained by backcrossing pf28, pf30, ssh1 with wild type.
LeDizet M, Piperno G (1995) The light chain p28 associates with a subset of inner dynein arm heavy chains in Chlamydomonas axonemes. Mol Biol Cell. 6:697-711
Kubo T, Hirono M, Aikawa T, Kamiya R, Witman GB (2015) Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Mol Biol Cell. 26:2810-22
CC-5258 pf23 tpg2-3
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation, lacks inner arm dynein a, c, and f, reduced outer arm dynein; short flagella
This mutant was obtained by crossing pf23 with ssh1 / tpg2-3
LeDizet M, Piperno G (1995) The light chain p28 associates with a subset of inner dynein arm heavy chains in Chlamydomonas axonemes. Mol Biol Cell. 6:697-711
Kubo T, Hirono M, Aikawa T, Kamiya R, Witman GB (2015) Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Mol Biol Cell. 26:2810-22
CC-5259 pf28 pf30 tpg2-3
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation, and lacks outer arm dynein and inner arm dynein f/I1; complete immotile
This mutant was isolated as a strain that suppresses the flagellar shortness of pf28, pf30.
LeDizet M, Piperno G (1995) The light chain p28 associates with a subset of inner dynein arm heavy chains in Chlamydomonas axonemes. Mol Biol Cell. 6:697-711
Freshour J, Yokoyama R, Mitchell DR (2007) Chlamydomonas flagellar outer row dynein assembly protein ODA7 interacts with both outer row and I1 inner row dyneins. J Biol Chem. 282:5404-12
Kubo T, Hirono M, Aikawa T, Kamiya R, Witman GB (2015) Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Mol Biol Cell. 26:2810-22
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: Lacks outer arm dynein and inner arm dynein f/I1; palmelloid (aflagellate phenotype)
This mutant was obtained by backcrossing pf28, pf30, tpg2-3 with wild type.
Kubo T, Hirono M, Aikawa T, Kamiya R, Witman GB (2015) Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Mol Biol Cell. 26:2810-22
CC-5261 ida4 pf3
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: lacks inner arm dynein a,c, and d, and dynein regulatory complex 1; short flagella
This mutant was obtained by crossing pf3 and ida4.
Kubo T, Yagi T, Kamiya R (2012) Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex. Cytoskeleton. 69:1059-68
CC-5262 ida4 pf3 tpg1
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation, lacks inner arm dynein a,c, and d, and dynein regulatory complex 1; generates wild-type length flagella, impaired motility
This mutant was obtained by crossing pf3,ida4 and tpg1.
Kubo T, Yagi T, Kamiya R (2012) Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex. Cytoskeleton. 69:1059-68
CC-5263 ida4 ida5 tpg1
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation, lacks inner arm dynein a,c, and d, and e; generates wild-type length flagella, impaired motility
This mutant was obtained by crossing ida4,ida5 and ida4,tpg1.
Kubo T, Yagi T, Kamiya R (2012) Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex. Cytoskeleton. 69:1059-68
CC-5264 ida4 oda2 tpg1
$30.00
$30.00
From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016
Phenotype: reduced axonemal tubulin polyglutamylation, lacks inner arm dynein a,c, and d, and e; generates wild-type length flagella, complete immotile
This mutant was obtained by crossing ida4 and oda2, tpg1.
Kubo T, Yagi T, Kamiya R (2012) Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex. Cytoskeleton. 69:1059-68
CC-5265 cdkg1-2 mt+ [44d8]
$30.00
$30.00
From Sa Geng, Jim Umen Lab, Danforth Plant Science Center, July 2016
This mutant was generated by an insertional mutagenesis screen with a NIT1-marked linearized plasmid pMN56 into CC-2453 (nit1-305 mt-), followed by backcrossing into a nit1-305 mt+ strain.
Li Y, Liu D, López-Paz C, Olson BJ, Umen JG (2016) A new class of cyclin dependent kinase in Chlamydomonas is required for coupling cell size to cell division. Elife. Mar 25;5
CC-5266 GSP1 mt+
$30.00
$30.00
From Sa Geng, Jim Umen Lab, Danforth Plant Science Center, July 2016
Hamaji T, Lopez D, Pellegrini M, Umen J (2016) Identification and Characterization of a cis-Regulatory Element for Zygotic Gene Expression in Chlamydomonas reinhardtii. G3 (Bethesda). 6:1541-8
CC-5267 GSP1 mt-
$30.00
$30.00
From Sa Geng, Jim Umen Lab, Danforth Plant Science Center, July 2016
Hamaji T, Lopez D, Pellegrini M, Umen J (2016) Identification and Characterization of a cis-Regulatory Element for Zygotic Gene Expression in Chlamydomonas reinhardtii. G3 (Bethesda). 6:1541-8
From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016
Mutant description: HAP2-HA rescued mfus7
Background strain: Mfus7, fusion mutant
Plasmid transformation: HAP2-HA (YJ58) and NIT (pMN56), co-transformation
From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016
Mutant description: FUS1-HA in B215
Background strain: B215
Plasmid transformation: FUS1-HA and pSI103 co-transformation
From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016
Mutant description: HAP2ecto+TMD in 40D4
Background strain: 40D4
Plasmid transformation: HAP2ecto+TMD plasmid with aphVIII in one plasmid
Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71
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